Question

If you treat intact chromatin (30 nm fiber) with heparin, to remove H1, followed by extensive treatment with a single restriction enzyme, what sort of banding pattern would you expect to see on a gel following de-proteinization and electrophoresis?

Answer 1

heparin unfolds chromatic and increases accessibility to Mn ase digestion. Heparin binds tightly to histone tails and they changed the net charge of the nucleosome it in he with binding of nucleosome containing complexes to other components. In the presence of heparin nucleosomes migrate faster in the other Roswell with migration rate corresponding to that of Mono nucleosomes. Increasing amount of heparin increased the Kinetics of DNA degradation resulting in complete degradation of nucleosomal DNA after few seconds.