You are studying myosin V, a class of myosin which can be used in cells to...

You are studying myosin V, a class of myosin which can be used in cells to transport vesicles by “walking” along actin filaments, towards the plus ends of these filaments. You have identified a mutation in the gene for myosin V that affects its ATP binding site. This mutant myosin V (M) can still perform the entire cross-bridge cycle, but it takes much longer to hydrolyze ATP compared to a wild type myosin V (W).

a) Would the duration of the cross-bridge cycle be longer in M or W?Explain?

b) Would the duty cycle (the proportion of the cross-bridge cycle in which myosin is bound to actin) be longer in M or W?Explain?

c) How would this difference in duty cycle affect the ability of M to transport a vesicle along an actin filament? Explain.

Expert Solution

The duration of the cross bridge is greater or longer in M as compared to W. Head of the myosin linked or bind the actin and pulls the actin filaments towards inwards resulting in the contraction of muscles. This myosin has also the ATP hydrolysing property which helps to convert the ATP into ADP and organic phosphate with release of large amount of energy. This energy is used to detach the myosin from the actin and this results in the relaxation of the muscles . The mutant M has defective ATP binding site . Therefore , the hydrolysing of ATP does not takes place or it takes place at very slow rate . Therefore due to No or slow release of energy by the hydrolysis of ATP . The mutant M can still perform the entire cross bridge cycle phenomenon . But it occurs at a very slow rates results in the sluggish movement . Contraction of muscles takes place. Relaxation occurs only by release of energy that is released by the hydrolysis of ATP molecules. by the ATP binding site of the myosin. In the relaxed state of the muscles, the myosin and the actin are seperated. To seperate the myosin and actin from each other . Certain binding proteins binds to the myosin binding site on the actin . These proteins are known as Tropomyosin .
Hence , The cross bridge cycle will be longer in M .
Thanking you
Have a beautiful day ?✨
Please, Hit a Thumbs up ??

gladiator answered 1 year ago

Which type of MHC class molecules is found on all nucleated cells and is used to...

Which type of MHC class molecules is found on all nucleated cells and is used to communicate with cytotoxic T-lymphocytes? Which class is found only on APCs and is used to communicate with helper T-lymphocytes? Where does a lymphocyte typically encounter an antigen for the first time? Describe how both helper T-lymphocytes and cytotoxic T-lymphocytes are activated. Is a separate APC required for B-lymphocyte activation, or is a B-lymphocyte able to serve the role of an APC? What are the...

You are studying a transcriptional activator protein called CYK1, which activates cytokine genes in immune cells...

You are studying a transcriptional activator protein called CYK1, which activates cytokine genes in immune cells and has both a nuclear localization and a nuclear export signal and is normally found both in the nucleus and in the cytosol at comparable concentrations. CYK1 has a high-affinity binding partner in the nucleus (the binding partner is restricted to the nucleus). Upon activation of a certain signaling pathway, the binding protein partner is tagged for degradation. Explain the outcome of activation of...

You are interested in studying the effect of laptops in class on students’ performance. At the...

You are interested in studying the effect of laptops in class on students’ performance. At the beginning of the quarter, you randomly assign your 250 students to sit on either the left half or the right half of the lecture hall. Students on the left half are told to bring laptops to class and to take notes on their laptops. Students who sit on the right side of the room are required to write notes on paper. At the end...

You are studying a bacterial pathogen that can evade the host's immune system with ease. Which...

You are studying a bacterial pathogen that can evade the host's immune system with ease. Which of the following virulence factors would it most likely possess? A. Hemolysin B. Adhesins C. Coagulase D. Endotoxin

You are studying cells cultured from patient sample and determine that they contain a large number...

You are studying cells cultured from patient sample and determine that they contain a large number of blocked DNA replication forks compared to control cells. You hypothesize that the patient cells would display a reduced number of blocked replication forks if you treated with a drug that activates which of the following? give brief explanation to your choice l. Pre-replication complexes ll. Protein kinase transducers lll. Checkpoint sensor proteins. lV. CDKs (cyclin-dependent kinases)

Which of the following is a difference between the cells used for electrolysis of water and...

Which of the following is a difference between the cells used for electrolysis of water and electrolysis of molten sodium chloride? Select the correct answer below: A barrier separates the half-reactions in the cell carrying out electrolysis of molten sodium chloride but not in the cell carrying out electrolysis of water. Sulfuric acid is added to the cell carrying out the electrolysis of molten sodium chloride but not to the cell carrying out the electrolysis of water. Inert electrodes are...

21. Which of the following mechanisms is used by cells to insure that proteins are correctly...

21. Which of the following mechanisms is used by cells to insure that proteins are correctly localized to specific organelles, integrated into the plasma membrane or secreted? a. Proteins become glycosylated in the rough ER and Golgi and only proteins with sugar modifications are transported to their correct sub cellular location. b. Proteins encode a sequence that acts as a signal, such as a nuclear localization signal (NLS), to target them appropriately. c. Any protein being synthesized is immediately inserted...

If muscle myosin is non-processive, then how is it that we can contract our muscles and...

If muscle myosin is non-processive, then how is it that we can contract our muscles and hold them that way for a sustained period of time? That is, how can our muscles resist a counter force (for example a heavy object) and contract continuously when we lift it despite the fact that the motor protein, myosin II, is non-processive?

Define an inflammasome. How is it activated and in which cells of the body can it...

Define an inflammasome. How is it activated and in which cells of the body can it be activated in, when it is activated, what is the cellular response and how does that response contribute to inflammation.

Overview For this assignment, design and implement the methods for a class that can be used...

Overview For this assignment, design and implement the methods for a class that can be used to represent a quadratic equation. int main() has already been written for this assignment. It is available for download from Blackboard or by using the following link: http://faculty.cs.niu.edu/~byrnes/csci240/pgms/240pgm8.cpp All that needs to be done for this assignment is to add the class definition and method implementation to the above CPP file. The Quadratic class Data Members The class contains three data members: an integer...

Question