Question

In which one of the following chromatography technique proteins are fractionated according to the biologically important binding interactions that occur on protein surfaces ?

Select one:

a. Hydrophobic chromatography

b. Gel-filtration chromatography

c. Affinity chromatography

d. Ion-exchange chromatography

Answer 1

the correct answer is option c - affinity chromatography

Affinity chromatography is a technique of separating organic chemistry mixture based on a extremely specific interaction between antigen and antibody, enzyme and substrate, receptor and ligand, or protein and nucleic acid. affinity chromatography takes advantage of the biologically important binding reactions that occur on protein surfaces.

option A is wrong because hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity.

option B is wrong because Gel filtration chromatography, a sort of size exclusion chromatography, is used to either fractionate molecules and complexes during a sample into fractions with a specific size range, to get rid of all molecules larger than a specific size from the sample, or a mix of both operations.

option D is wrong because ion-exchange chromatography could be a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. It works on nearly any kind of charged molecule—including massive proteins, tiny nucleotides, and amino acids.