Why is the ability to separately detect different colors (ie. red, green, blue) so useful in...

Why is the ability to separately detect different colors (ie. red, green, blue) so useful in fluorescence microscopy? That is, what does this ability allow cell biologists to do?

Expert Solution

Flouroscence microscope can detect multiple colur probe ( green , blue etc..) separately. Thus it is possible to identify cells and various sub microscopic cellular components with a high degree specificity. By using different colur probe or multiple probe at a time we can detect multiple sub cellular structures or target molecules simultaneously in a cell. Along with this we can detect presence of two cellular target st a time in the cell using 2 fluorescent probe .

As individuals prove give signal for each target .

But if mixing is present in both probe this denotes that both target are located in the same compartment in the cell.

For example we are using stain for nucleus ( DNA ) as well as tubulin protein . Both are located in nucleus, by applying two fluorescent probes there will be mixing and generation of new signal along with individual fluorescent signal.

gladiator answered 2 years ago

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