Phenol red, a pH indicator, is used in the experiment to
determine respiration activity by showing CO2
production.
Table: CO2 Production by Beans
Beans
Indicator Color
(Phenol Red)
Conclusion
(CO2 present or absent)
Germinating
Yellow
Present
Ungerminated-dry
Red
Absent
Questions;
1. How does phenol red work as a pH indicator?
2. Why does this show respiration activity?
Mix the watery environment of Elodea with a pH
indicator called phenol red. In the presence of an acid, phenol red
will appear yellow. In the presence of a strong base, phenol red
will appear purple. Intermediate colors correspond with
intermediate pH values. Recall that plants use CO2 for
photosynthesis; the concentration of CO2 will determine
the pH of a solution. Plants also produce CO2 as a
byproduct of cellular respiration.
Will a solution with a high concentration of CO2...
Please write a brief description of each type of agar
(MacConkey's, Tryptic Soy Agar, Mannitol Salt Agar, and Blood Agar)
and it's purpose (what is in it, why? and what type of bacteria are
cultured with it when do you use each type?)
1.what kind of medium is Mannitol Salts Agar (MSA)
a) neither selective nor differential
b)differential
c)selective
d)both selective and differential
2.which of the following is the best method to distinguish
Bacillus megaterium from Bacillus Subtilis?
a)use mannitol phenol red
b)streak a MacConkey plate
c)perform an endospore stain
d) perform a gram stain
What can cause a phenol red indicator of carbohydrate
fermentation to give a false positive with gas?
I heavily incubated my organism in the test tube and it is
producing a huge gas bubble in the Durham tube and a clear yellow
(acid) color. I incubated my tube at my organism's optimism growth
conditions (oxygen and temperature wise) for 48 hrs exactly.. i was
supposed to get a red no gas result ( -) what might have gone
wrong?
4a. Describe why the EMB, Hektoen Enteric and Mannitol Salt
agar are selective. Include
specific selective ingredients added to each media and what
organisms these media select for and
against.
4b. Describe why the EMB, Hektoen Enteric and Mannitol Salt
agar are differential. Include
specific differential ingredients added to each media and
which types of organisms produce
positive differential results.
What is the purpose of using blood agar? What are hemolysins?
What are the three patterns of hemolysis on blood agar? Compare and
contrast alpha, beta, and gamma hemolysis. What are the hemolytic
patterns for a normal throat culture? What are the hemolytic
patterns for Streptococcus pneumoniae and Streptococcus
pyogenes?
What will happen in a phenol red test if you incubate an
obligate aerobe with glucose as the fermentable sugar?
What is the substrate for the catalase enzyme? What do you
detect if the catalase test is positive?
Why doesn't the iodine react with starch in the agar surrounding
an organism that produces amylase?
Experiment "preparation and standardization of NaOH
solution"
What is the purpose of an indicator soltion?
What does standardization mean? why does the NaOH solution have
to be standardized???
Calculae ho many grams of solid NaOH are needed to prepare 500mL
NaOH solution with a concentration of 0.4M (show work)
For your indicator, what is the wavelength of maximum absorbance
for the pH < 4.00 solution? What is the wavelength of maximum
absorbance for the pH > 10.00 solution? What colors correspond
to these wavelengths? How do the observed colors of these solutions
relate to the colors at their absorbance maxima? This is the
procedure:
http://www3.chem21labs.com/labfiles/UofC_GL18_Lab.pdf?rf=5204