One thousand bases of quail mitochondrial DNA were downloaded to Geneious from the GenBank database. PCR...

One thousand bases of quail mitochondrial DNA were downloaded to Geneious from the GenBank database. PCR primers were designed to amplify a conserved region from this sequence. Each primer was 20 bases long, and the 5` end of the forward primer (F) and reverse primer (R) starts from base position 33 and 865, respectively. A PCR master mixture for 23 reactions to analyse different bird samples was prepared.
1. Fill in the necessary information for the master mix in the following table

No of reactions to make up			23
Vol of each Reaction (µL)			24 µL +1 µL DNA
Total Volume Required			_____µL total
Component	Supplied as	Final Conc inPCR	µL required 1rxn 23 MgCl2 rxn’s
MgCl2	25 mM	2.5 mM
Polymerase buffer	5x	1x
Primer forward (F)	10 µM	1 µM
Primer Reverse (R)	10 µM	1 µM
Taq DNA polymerase	1Units/µL	1 U per rxn
dNTPs	25 mM	1 mM
Template DNA added later	-	-
Water	-	-
		Total volume	25 µL

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gladiator answered 1 year ago

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