In: Biology
No of reactions to make up |
23 |
|||
Vol of each Reaction (µL) |
24 µL +1 µL DNA |
|||
Total Volume Required |
_____µL total |
|||
Component |
Supplied as |
Final Conc inPCR |
µL required 1rxn 23 MgCl2 rxn’s |
|
MgCl2 |
25 mM |
2.5 mM |
||
Polymerase buffer |
5x |
1x |
||
Primer forward (F) |
10 µM |
1 µM |
||
Primer Reverse (R) |
10 µM |
1 µM |
||
Taq DNA polymerase |
1Units/µL |
1 U per rxn |
||
dNTPs |
25 mM |
1 mM |
||
Template DNA added later |
- |
- |
||
Water |
- |
- |
||
Total volume |
25 µL |