1) Why do peaks not occur as a simple line? In other words, why is the...

1) Why do peaks not occur as a simple line? In other words, why is the peak brought at the bottom and narrow at the top of the peak? This is referring to GC analysis

Expert Solution

The ideal chromatographic system should produce peaks that are straight line spikes with no broadening. Though, there are two main reasons why broadening in experienced in the chromatographic peaks.

1) Dead volume: It refers to all the volume in a chromatographic system from the injector to the detector other than the column. Since separation occurs only in the column, all other volumes (tubing using to connect components, volume within the detector cell, etc) have the ability to contribute to peak broadening but not to the separation.

2) Column: The other source of broadening is within the column. There are several features of the design of the column that leads to broadening in the chromatographic peak. There are four general contribution to broadening within chromatographic columns:

(i) longitudinal diffusion.

(ii) eddy diffusion.

(iii) mass transport broadening in stationary phase.

(iv) mass transport broadening in mobile phase.

queen_honey_blossom answered 2 years ago

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