Question

In mRNA splicing, among other proteins, these proteins are involved to : Cwc22, Snu114, MIF4G, and RecA1. Please explain their relationship and their function in RNA splicing. Are there other names for these proteins that are better known.

Answer 1

CWC22 - Also Known as Human Spliceosomal protein

MIF4G - Also Known as Dead box Protein

Rec A1 - Also known as Recombinase A1

Snu114 - Also known as Pre mRNA splicing factor

Snu114, a component of the U5 snRNP, plays a key role in activation of the spliceosome. It controls the action of Brr2, an RNA-stimulated ATPase/RNA helicase that disrupts U4/U6 snRNA base-pairing prior to formation of the spliceosome's catalytic centre.

MIF4G are involved in all aspects of RNA processing. They bind RNA in an ATP-dependent manner and couple ATP hydrolysis to structural and compositional rearrangements of ribonucleoprotein particles.

CWC22 plays a role in coupling splicing to exon junction complex deposition and nonsense-mediated decay. ... Nonetheless, hCWC22 depletion yields increased levels of spliced RNA from the unusual nonsense codon-containing U22 host gene, which is a natural substrate of NMD.

RecA1 protein can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.