Question

The PCR reaction will be carried out with the following cycle conditions: 95 degrees C for 45 seconds, 65 degrees C for 45 seconds, and 72 degrees C for 1 minute 45 seconds. Which of these three temperatures represents the annealing step?

72? degrees  

The minimal amount of time required for one of these steps varies depending upon the D1S80 allele that is being amplified. Which of these three temperatures represents this step?

____ degrees

Answer 1

Hi Answer:

Q. The PCR reaction will be carried out with the following cycle conditions: 95 degrees C for 45 seconds, 65 degrees C for 45 seconds, and 72 degrees C for 1 minute 45 seconds. Which of these three temperatures represents the annealing step?72?degrees

Answer: Every PCR reaction have following main steps i.e. Denaturation (94-98oC), Annealing (50-65^oC), extension (70-80^oC), Final elongation (70-74^oC), and Final hold (4-15^oC). In the above-given temperatures, 65^oC is the annealing temperature.

Q. The minimal amount of time required for one of these steps varies depending upon the D1S80 allele that is being amplified. Which of these three temperatures represents this step?____ degrees

Answer: The amplification of the DNA took place at the third step i.e. extension step, in this step the primer attached to the denatured strands of DNA start building the DNA building blocks which are complementary to the main template which is associated with the primer, making a double-stranded DNA molecule. In above-given temperature, the third temperature i.e. 72^oC for 1min 45 sec. is the extension step temperature.