Question

Currently a lot of research is happening about the corona virus. What type of a laboratory should be used to conduct these investigations and why?

Answer 1

Solution

Lab should have

• Lab should contains molecular (RT-PCR) and serological testing facility.
• Using automated instruments and analyzers
• Processing initial samples
• Staining and microscopic analysis of fixed smears
• Examination of bacterial cultures
• Pathologic examination and processing of formalin-fixed or otherwise inactivated tissues
• Molecular analysis of extracted nucleic acid preparations
• Final packaging of specimens for transport to diagnostic laboratories for additional testing (specimens should already be in a sealed, decontaminated primary container)
• Using inactivated specimens, such as specimens in nucleic acid extraction buffer
• Performing electron microscopic studies with glutaraldehyde-fixed grids

This is required for

• precautions should be taken in handling specimens that are suspected or confirmed for SARS-CoV-2.
• For procedures with a high likelihood to generate aerosols or droplets, use either a certified Class II Biological Safety Cabinet (BSC) or additional precautions to provide a barrier between the specimen and personnel.
• Examples of these additional precautions include personal protective equipment (PPE), such as a surgical mask or face shield, or other physical barriers, like a splash shield; centrifuge safety cups; and sealed centrifuge rotors to reduce the risk of exposure to laboratory personnel.
• Virus isolation in cell culture and initial characterization of viral agents recovered in cultures of SARS-CoV-2 specimens should only be conducted in a Biosafety Level 3 (BSL-3) laboratory using BSL-3 practices.
• Decontaminate work surfaces and equipment with appropriate disinfectants. Use EPA-registered hospital disinfectants with label claims to be effective against SARS-CoV-2external icon. Follow manufacturer’s recommendations for use, such as dilution, contact time, and safe handling
• Laboratory waste managment and safety precaution required to stop infection at lab level.