Question

Explain the relationship between the measurement of 2-deoxyadenosine and how this is being used to determine the amount of DNA in the experiment that uses diphenylamine reaction

Answer 1

2-DEOXYADENOSIN(Purine)::-- it is derivative of nucleocide Adenosine, differing from the latter in case of OH in which In place of OH, H will be thereat 2'position. It is composed of adenine attached to deoxyribose moiety via a N9-glycocidic bond. This is ''A" in DNA which pairs with deoxythimidine (T) in DNA.

Now come to the point of relation between 2 deoxyadenisine(Purine) and its used in amount of DNA.::--

Now lets now the reaction of diphenylamine with DNA and it will show us that how it(deoxyadenosine) is being used here::--

Reaction is as follows::-

*Will form blue coloured compound.

* Treated under high acidic environment cause depurination of DNA and dehydrate it into reactive hydroxylevulinylaldehyde. As this deoxyribose sugar link to only purin residues(deoxyadenosin or deoxythimidine) participates in the reaction, the readout is only from 50% of the the total nucleotide .

So this number helps in estimation of DNA and in such a way deoxyadenoaine(purine)link to sugar participates and helps in know the amount of DNA and a graph is plotted on the basis of it in estimation of DNA.

That it.