Question

In: Biology

Escherichia coli Pseudomonas malli Bacillus megaterium Bacillusanthracis, Lactobacillusacidophilus, Pseudomonas aeruginosa, Serratia marcescens Staphylococcus caprae Streptococcus mutans...

Escherichia coli

Pseudomonas malli

Bacillus megaterium

Bacillusanthracis,

Lactobacillusacidophilus,

Pseudomonas aeruginosa,

Serratia marcescens

Staphylococcus caprae

Streptococcus mutans

Neisseria gonorrhoeae,

Staphylococcus aureus,

Streptococcus pyogenes,

Proteus myxofaciens,

Klebsiella pneumoniae sub sp pneumoniae

Salmonella bongrori

Shigella sonnei

Corynebacterium xerosis

Enterobacter aerogenes

Proteus vulgaris

Escherichia vulneris

Escherichia blattae

Salmonella choleraesuis (subsp. diarizonae)

Enterobacter hormaechei

Enterobacter asburiae

Citrobacter diversus

Citrobacter freudii

In order to receive full credit for this assignment, you must provide the following;

The correct identification of Unknown organism in this homework assignment.

The correct interpretation of the biochemical tests in the table below for this unknown organism.

Create a flow chart which demonstrates the tests that allowed you separate the unknown organism from the list.

Use Microsoft PowerPoint, it is easier for creating flow charts compared to Microsoft Word

You do not need to isolate the entire list of organisms from each other, just your unknown from the list. See the sample flow chart.

Using your biochemical and morphological results to indentify the identity of your orphan organism by using the Bergey’s manual.

Create a flow chart and identify your orphan organism.

UNKNOWN # 3

The follow biochemical tests were performed after an unknown bacterium was isolated into a pure culture. Each of the tests was incubated at 37°C for 24 hours and when appropriate the use of reagents was added in the correct volumes to each of the tests. The following table describes these results.

      

      

Name of the Biochemical Test/ Media

Appearance after addition of the reagent

POS/NEG

Interpret the Resulting Reactions of the test

( POS or NEG) 1 pts each

Catalase (example)

Bubbles

POS

POS for catalase/ H2O2  àH20 + O2

Urea/ Urea broth

Reddish / yellow

Phenol red Lactose

Red

Phenol red Mannitol

Yellow

Simmons Citrate

Blue

Methyl red (MR)/ MRVP broth

Red

Vogus Proskauer (VP)/MRVP broth

No change/copper color

Indole production

/ SIM media

No red ring

Phenol red Glucose

Yellow

Gram Stain

Pink

FTM tube/ fluid thioglycollate media

Growth though out the tube

0.4% Agar with Nutrient broth and1% TTC.

Growth and Red though out the tube

Starch Hydrolysis/ Starch agar

Brown around the inoculation

Gelatin Hydrolysis/ Gelatin media

Solid

Oxidase

No color change

Nitrate reduction/ Nitrate broth

Red After Nitrate A&B reagent is added

Shape

ROD

Fat hydrolysis/ Spirit blue agar

White around the inoculation

H2S production/ SIM media

Black

Solutions

Expert Solution

Urea - Reddish yellow - negative

Phenol red - Red - negative

Phenol red mannitol - positive - change in pH of the medium

Simmons citrate - Blue - positive - change in pH causes color change

Methyl red - Red - positive - mixed acid fermnetation

Vogus proskauer - no change - negative - acetyl methyl carbinol is absent

Indole - No ring - negative - no indole production

Phenol red glucose test - Yellow - positive - fermentation , changespH  

gram stain - pink - gram negative

FTM tube - throughout growth -facultatively anerobe

0.4% agar ... (motility test ) - Non motile

starch hydrolysis - positive - amylase production

Gelatin hydrolysis - negative - no gelatinase production

Oxidase - negative - no oxidase production

Shape - Rod

Fat hydrolysis - positive - hydrolysis of fat

H2s - black precipitate - positive - H2s production.

  • From the results , Proteus myxofaciens is the unnown organism from the given list , which is compatible with the test results

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