Question

What are the goals of the Biology Gen III microplate and how can you determine an outcome from the basis that can be observed. How are bacteria added and how is growth measured in the Biology Gen III? Likewise, how can the selected nutrients and the growth patterns in the Gen III microplate be used to identify common clinical and industrial strains of bacteria quickly?

Answer 1

goal

this is used to identify an unknown bacterium. it is used to Analyses the Ability of the cell to metabolize all class of biochemicals. also can determine chemical sensibility, reducing power, and ph ad acid tolerance.

as per the universal protocol, the outcomes are determined by computer systems. using the micro station to read the microplate and also make species identification. there are full , semi ans manual systems to Analyses the microplates. providing software requirements and checking the microplates before loading provide you the est analysis by this system. incubation is done at 33 degree celsius. the cels must be fresh. incubation period is 4-24 hours foe almost all organisms. to minimize sporulation some bacterias are incubated 16 hours. usually media , bacterial solution and dye are added to the plates.

by this technology, we can do 94 phenotypic tests. 71 re carbon source utilization assay and 23 chemical assay. the results provide phenotypic fingerprint which can be used to identify it to the species level of microorganism. this finger printing helps to identify the newly found bacterias from the stored data. characterization of organisms can be done and store by this technique.