You plan on isolating a protein of interest by cutting a band out of a gel. Your protein is a phosphatase (a certain type of enzyme) and you hope to measure its activity. Should you use SDS-PAGE or a native gel to isolate your protein? Why?

Discuss the relationship between the location of an atom in the periodic table and its electron configuration.

List and describe the significance of the quantum numbers needed to specify the internal state of hydrogen-like atoms.

In a hydrogen atom, how might an electron move from one energy level to another?

Ligand X forms a complex with both cobalt and copper, each of which has a maximum absorbance at 510 nm and 645 nm, respectively. A 0.263-g sample containing cobalt and copper was dissolved and diluted to a volume of 100.0 mL. A solution containing ligand X was added to a 50.0 mL aliquot of the sample solution and diluted to a final volume of 100.0 mL. The measured absorbance of the unknown solution was 0.546 at 510 nm and 0.399 at 645 nm, when measured with a 1.00-cm cell. The molar absorptivities of the cobalt and copper complexes at each wavelength are shown in the table below.

What is the concentration of cobalt and copper in the final diluted solution?

What is the weight percent of cobalt (FM = 58.933 g/mol) and copper (FM = 63.546 g/mol) in the 0.263-g sample?

a. If a Bragg angle of 41.31^o 2-theta is observed for a first order peak from a {110} plane in a pure BCC element using Cu K-alpha radiation (1.54 A wavelength), what is the interplanar spacing of the {110} planes in this material?

b. What is lattice parameter of this element based on this peak?

c. If the density were measured to be 5.8 g/ml, how could you use the lattice parameter value calcuated in part B to determine which element this is using only a perodic table.

The Kb for an amine is 2.105 × 10-5. What percentage of the amine is protonated if the pH of a solution of the amine is 9.788? (Assume that all OH– came from the reaction of B with H2O.)

A mixture initially contains A, B, and C in the following concentrations: [A] = 0.300 M , [B] = 1.30 M , and [C] = 0.400 M . The following reaction occurs and equilibrium is established: A+2B⇌C At equilibrium, [A] = 0.170 M and [C] = 0.530 M . Calculate the value of the equilibrium constant, Kc.

Describe the challenge that rooftop solar energy presents to electric grid operators in California.

Part A) 525mL of 1.50M NaCl is added to a beaker containing 378mL of 2.22M AgNO3, resulting in the formation of AgCl precipitate. What is the limiting reactant?

NaCl

AgNO3

AgCl

NaNO3

Part B) How many grams of K₂SO₄ are in 250mL of 0.11 M K₂SO₄ solution?

___ g K₂SO₄

Part C) The reaction of magnesium nitride and water is shown below:

Mg₃N₂ + 3H₂O --> 2NH₃ + 3MgO

During a lab experiment, 3.82 g magnesium nitride and 7.73 g water are placed in a reaction flask.

At the end of the experiment, the amount of magnesium oxide produced is 3.60 g.

What is the percent yield of the reaction?

Explain why atomic emission spectroscopy can be used for multi-elemental analysis, but atomic absorption is used mostly for quantification of single element.

Phosphorous acid, H3PO3(aq), is a diprotic oxyacid that is an important compound in industry and agriculture.

Calculate the pH for each of the following points in the titration of 50.0 mL of a 1.5 M H3PO3(aq) with 1.5 M KOH(aq).

pKa1= 1.30

pKa2= 6.70

(a) before addition of any KOH =_____number

(b) after addition of 25.0 mL of KOH = ______number

(c) after addition of 50.0 mL of KOH = ______number

(d) after addition of 75.0 mL of KOH =_______number

(e) after addition of 100.0 mL of KOH=______number

please explain how you got each! thanks so much

also this might help: For part (a), you only need to consider the first ionization because Ka1 >> Ka2. Use pKa1 to find the value of Ka1, then solve for x.

Calculate the volume of 0.20 M NaOH required to bring the pH of 20 mL of 5% acetic acid to 4.74, the pKa of acetic acid.

Please and thank you in advance :)

Consider a tank initially containing 3L of 1M HCl. To this tank, we add 3L of 2M Ca(OH)₂, and allow the acid-base reaction to proceed to completion.

(a) At the end of this step, what are the concentrations of HCl, Ca(OH)₂ and CaCl₂ in the mixture ? (Please note that there is water generated during the reaction)

We then remove 2L of the contents of the tank, add 2L of 0.5M HCl, and allow the reaction (if any) to proceed to completion

(b) what are the concentrations of HCl, Ca(OH)₂ and CaCl₂ in the mixture now ? (Again, note that water may be generated due to the reaction)

[NB: You may assume that all of the above occurs at a temperature where the density of water is 1000 kg/m³ (1000 g/L)]

Please show work! I've missed class due to illness so I need to know how to do this.

Assign the molecules CCl₂CCl₂, CCl₂CF₂, SeO₃, PCl₃, C₂O₄^2-, CH₃Cl, CH₃CH₂Cl,SO to point groups with explantion.