Question

Short explanatory notes on decalcification provess.

Answer 1

Declaficaiton is the process of removing calcium from the tissue. calcified tissue should be decalficied before processing. using experienced technique piece of tissue properly decalcified. different tissue types need different tissue in the decacifying solution depending on the size and density of the tissue. this process need monitoring over several hours or days. strat decalficaiton at the beginning of the week not on the weekend for maintaining constant observation of the tissue.
Remove the excess tissue from around the bone if tissue is not needed it help for better penetration of decalcifying solution into the tissue.fix the tissue completely and remove tissue from fixative and rinse in the tap water. place the tissue in 10% formic acid solution till tissue determined to be decalcified. this process take several hours to days. use large amount of solution to tissue and change the solution daily if process take more than one day. remove tissue from formic acid and rinse in running tapwater for ten minutes. place the tissue in 70% ethanol for storage and delivery.