Question

Which of the following is INCORRECT when considering how to identify the successful incorporation of a DNA insert into a vector?

A. Colony PCR indicates the presence and size of an insert

B Ampicillin resistance indicates the presence of an insert

C Blue/white screening indicates the presence of an insert

D DNA sequencing confirms the identity of a correct insert

A mutation in the iron response element (IRE)-binding protein causes it to have a much higher affinity for the IRE compared to iron. Predict the most likely outcome of this mutation:

A Cellular iron levels will rapidly increase

B Transcription will be blocked if the IRE is located upstream of the protein-coding region

C Translation of all IRE-containing transcripts will be inhibited

D Translation of iron storage proteins will proceed via a 5' cap-independent pathway

A matrix protein, ELN, is highly expressed in skin cells, but not expressed at all in liver cells. The ELN protein is encoded by the eln gene. Which of the following most likely accounts for tissue-specific ELN expression?

A The eln gene has a stronger promoter in skin cells than in liver cells

B A transcription factor needed for ELN expression may be missing in liver cells

C The RNA polymerase II may be mutated in liver cells

D The eln gene may lack an enhancer region in liver cells

Answer 1

Answer - option number b is the answer.

Which state that the Ampicillin resistance indicates the presence of an insert.

This statement it is incorrect because ampicillin resistance can be an indication of transformation or or we can say a get the plasmid containing the the ampicillin resistant gene is there in the bacteria but it is not an indication of presence of insert.

But the remaining options are correct because the blue white screening, colony PCR and sequencing method can be utilised to find or to confirm the presence of insert.

Answer -

Option number be is the answer

Which states that transcription factor needed for ELN expression may be missing in liver cells

This is because the expression of a particular gene depend on the presence of the regulators of that gene which regulate the the expression of that gene. In every cell we have all the gene but specific cell express only specific protein which give them unique Identity to the cell and the expression of specific gene is regulated by their specific regulator which are found specifically only in the specific gene expressing cells.

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