Question

What are the functions of RecF protein in homologous replication/repairing DNA? STEP BY STEP. Explain like you are explaining to a child.

Answer 1

ANSWER :-

Homologous recombination is a type of genetic recombination in which the nucleotide sequence are exchanged between the two sister chromatids and Homologous recombination repair is a DNA repair mechanism in which the invasion of undamaged DNA molecule by a damaged molecule of identical in a very similar sequence.

For the process of homologous recombination repair, several proteins is being required like, RecF protein, Rec Q, etc.

The function of RecF protein is to repair the DNA in the RecF pathway. The RecF pathway, also called the RecFOR pathway, is a mechanism or pathway of homologous recombination that repairs the DNA in organism. It repairs the breaks that occur on only one of DNAs two strands, known as single-strand gaps. This RecF protein also repairs the double-strand break(DSB) in DNA when the RecBCD protein is inactivated by mutation. The RecBCD pathway is another pathway of homologous recombination. The RecF protein requires the RecA protein for strand invasion. The RecF pathway perform the phrase of branch migration, in which the holiday junction slides in one direction, and resolution, in which the holiday junctions are cleaves apart by enzymes.

The RecF pathway begins when RecJ , an exonuclease that cleaves single-stranded DNA into 5'-3' direction binds to 5' end of single-strand gap in DNA and starts moving upstream while cleaving the 5' strand. .