nclude gross morphology and significant media; gram stain morphology, benchside testing, and biochemical testing and any...

nclude gross morphology and significant media; gram stain morphology, benchside testing, and biochemical testing and any appropriated automated systems

organism – Staphylococcus saprophyticus

include a gross morphology, bench testing and biochemical testing for the organism posted above

what information

Expert Solution

Solution

Organism: Staphylococcus saprophyticus ( Coagulase-Negative Staphylococcus or CoNS)

Gross morphology:

Shape	Spherical cocci, forms grape-like clusters, can occur single, in pairs, tetrads or short chains
Size	0.5-1.5 um
Motility	Non-motile
Capsule	Encapsulated
Spore	Non-sporulating
Gram-Stain	Positive
Colony Morphology	Dwarf colonies, smooth, glossy, butyrous and sometimes wet appearance. Opaqe and white/non-pigmented in color.

Cuture Media: Phosphate blood agar or MacConkey agar

Antibiotic resistance: Novobiocin

Bench testing: The bench testing procedures can include colony charatierization, Gram-testing, Catalase test etc.

Biochemical testing to identify S. saprophyticus:

Test	Result
Catalase	Positive
Coagulase	Negative
DNase	Negative
Glucose	Negative
D-Xylose	Negative
Sucrose	Positive
D-Trehalose	Positive
Maltose	Positive
Anaerobic growth in thioglycollate	Positive
Urea	Positive

Appropriated automated system used for detection: Vitek automated anitmicrobial identification and susceptibility testing systems. The card that can be used in this case would be GPI (Gram Positive Identification) card.

gladiator answered 1 year ago

SUBMIT AN ALGORITHYM/FLOWCHART FOR Staphylococcus saprophyticus ORGANISMS. INCLUDE GROSS MORPHOLOGY ON PRIMARY AND SIGNIFICANT MEDIA; GRAM...

SUBMIT AN ALGORITHYM/FLOWCHART FOR Staphylococcus saprophyticus ORGANISMS. INCLUDE GROSS MORPHOLOGY ON PRIMARY AND SIGNIFICANT MEDIA; GRAM STAIN MORPHOLOGY (AND ARRANGEMENT WHEN SIGNIFICANT), BENCHSIDE TESTING, AND BIOCHEMICAL TESTING USED FOR IDENTIFICATION TO THE GENUS/SPECIES LEVEL. USE THE IDENTIFICATION CHARTS FROM GRAM POSITIVE POWERPOINTS AND THE GRAM NEGATIVE CHARTS I POSTED IN THE LAB HANDOUT FOLDER AS A GUIDE. BE CREATIVE. FOR THE GRAM NEGATIVES YOU SHOULD BE ABLE TO GIVE BIOCHEMICAL CHARACTERISTICS OF THE SPECIES. I REALIZE YOU WILL NOT HAVE API'S AVAILABLE TO...

SUBMIT A FLOWCHART FOR acinetobacter baumanni organism .Also, INCLUDE GROSS MORPHOLOGY ON PRIMARY AND SIGNIFICANT MEDIA;...

SUBMIT A FLOWCHART FOR acinetobacter baumanni organism .Also, INCLUDE GROSS MORPHOLOGY ON PRIMARY AND SIGNIFICANT MEDIA; GRAM STAIN MORPHOLOGY (AND ARRANGEMENT WHEN SIGNIFICANT), BENCHSIDE TESTING, AND BIOCHEMICAL TESTING USED FOR IDENTIFICATION TO THE GENUS/SPECIES LEVEL. USE THE IDENTIFICATION CHARTS FROM GRAM POSITIVE POWERPOINTS AND THE GRAM NEGATIVE CHARTS I POSTED IN THE LAB HANDOUT FOLDER AS A GUIDE. BE CREATIVE. FOR THE GRAM NEGATIVES YOU SHOULD BE ABLE TO GIVE BIOCHEMICAL CHARACTERISTICS OF THE SPECIES. I REALIZE YOU WILL NOT...

What is Mycobacterum tuberculosis, and what is the morphology, gram stain characteristics, virulence factors, susceptibility to...

What is Mycobacterum tuberculosis, and what is the morphology, gram stain characteristics, virulence factors, susceptibility to antibiotics, host cells, nutritional needs, growth conditions, mechanisms used to evade the immune system and invasion into the host(s), interactions with the hosts and diseases caused and affected body systems. Additionally, students should explain symptoms when the Mycobacterum tuberculosis infects a host, as well as a diagnosis and the therapeutic intervention needed after infection. You may also add information on statistics related to infection...

1, Briefly discuss how the Gram stain works, Not the steps but the biochemical basis, what...

1, Briefly discuss how the Gram stain works, Not the steps but the biochemical basis, what leads to the differential staining? Name one organism (genus and species) that is a Gram positive coccus, and one that the Gram stain will not work on. 2, Choose 2 of these diagnostic tests and briefly describe how each works. Your answer should include what is used and what a positive result would look like. Catalase test, hemagglutinin assay, coagulase test, IF test, virus...

The pathogen I chose was Malaria, what is the morphology, gram stain characteristics, virulence factors, susceptibility...

The pathogen I chose was Malaria, what is the morphology, gram stain characteristics, virulence factors, susceptibility to antibiotics, host cells, nutritional needs, growth conditions, mechanisms used to evade the immune system and invasion into the host(s), interactions with the hosts and diseases caused and affected body systems for this pathogen. Also explain symptoms when the pathogen infects a host, as well as a diagnosis and the therapeutic intervention needed after infection. Information on statistics related to infection (epidemiology) and any...

1. Why Staphylococcus epidermis has gram stain positive? and why its cell morphology look like staph???

The Gram stain differentiates Gram + bacteria from Gram – bacteria because of which of the...

The Gram stain differentiates Gram + bacteria from Gram – bacteria because of which of the following? Group of answer choices presence of an outer membrane in one group and absence in the other presence of teichoic acid in one group and absence in the other presence of ether-linked lipids in one group and absence in the other presence of peptidoglycan in one group and absence in the other difference in thickness of peptidoglycan layer between the two groups

"The most widely used stain in microbiology is the Gram stain. Based on differences in the...

"The most widely used stain in microbiology is the Gram stain. Based on differences in the structure of the bacterial cell wall, the Gram stain divides bacteria into two major groups: Gram-positive and Gram-negative. Gram-positive cells have a thick layer of peptidoglycan, a polymer made of amino acids and sugars, in the cell wall. Crystal violet binds to peptidoglycan, rendering the cell purple. Gram-negative cells also have peptidoglycan and initially also stain purple. Since the peptidoglycan layer is much thinner,...

1. In gram stain if at the end of the procedure both the Gram-positive and Gramnegative...

1. In gram stain if at the end of the procedure both the Gram-positive and Gramnegative bacteria appear red what was the most common Technical error 2. In Gram stain you stop the procedure atter decolonization how would the Gram-negative bacteria appear? How would the Gram-positive

7) List the steps of the Gram stain procedure

Question