nclude gross morphology and significant media; gram stain morphology, benchside testing, and biochemical testing and any...

nclude gross morphology and significant media; gram stain morphology, benchside testing, and biochemical testing and any appropriated automated systems

organism – Staphylococcus saprophyticus

include a gross morphology, bench testing and biochemical testing for the organism posted above

what information

Expert Solution

Solution

Organism: Staphylococcus saprophyticus ( Coagulase-Negative Staphylococcus or CoNS)

Gross morphology:

Shape	Spherical cocci, forms grape-like clusters, can occur single, in pairs, tetrads or short chains
Size	0.5-1.5 um
Motility	Non-motile
Capsule	Encapsulated
Spore	Non-sporulating
Gram-Stain	Positive
Colony Morphology	Dwarf colonies, smooth, glossy, butyrous and sometimes wet appearance. Opaqe and white/non-pigmented in color.

Cuture Media: Phosphate blood agar or MacConkey agar

Antibiotic resistance: Novobiocin

Bench testing: The bench testing procedures can include colony charatierization, Gram-testing, Catalase test etc.

Biochemical testing to identify S. saprophyticus:

Test	Result
Catalase	Positive
Coagulase	Negative
DNase	Negative
Glucose	Negative
D-Xylose	Negative
Sucrose	Positive
D-Trehalose	Positive
Maltose	Positive
Anaerobic growth in thioglycollate	Positive
Urea	Positive

Appropriated automated system used for detection: Vitek automated anitmicrobial identification and susceptibility testing systems. The card that can be used in this case would be GPI (Gram Positive Identification) card.

gladiator answered 2 years ago

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