Question

SUBMIT AN ALGORITHYM/FLOWCHART FOR Staphylococcus saprophyticus ORGANISMS. INCLUDE GROSS MORPHOLOGY ON PRIMARY AND SIGNIFICANT MEDIA; GRAM STAIN MORPHOLOGY (AND ARRANGEMENT WHEN SIGNIFICANT), BENCHSIDE TESTING, AND BIOCHEMICAL TESTING USED FOR IDENTIFICATION TO THE GENUS/SPECIES LEVEL.  USE THE IDENTIFICATION CHARTS FROM GRAM POSITIVE POWERPOINTS AND THE GRAM NEGATIVE CHARTS I POSTED IN THE LAB HANDOUT FOLDER AS A GUIDE.  BE CREATIVE. FOR THE GRAM NEGATIVES YOU SHOULD BE ABLE TO GIVE BIOCHEMICAL CHARACTERISTICS OF THE SPECIES.  I REALIZE YOU WILL NOT HAVE API'S AVAILABLE TO SUBMIT BUT THERE SHOULD BE ENOUGH INFO TO LEAD TO A PROPER IDENTIFICATION. GIVE ME AS MUCH AS YOU CAN - GET CREATIVE - RESEARCH

Answer 1

•Let us understand first the genus Staphylococcus.it is a genus of Gram-positive bacteria in the family Staphylococcaceae in the order Bacillales. Under the microscope, they appear spherical (cocci), and form in grape-like clusters. Staphylococcus species are facultative anaerobic organisms and includes at least 40 species.

•Let us focus on Staphylococcus saprophyticus :- It is a Gram-positive, coagulase negative, non-hemolytic coccus that is a common cause of uncomplicated urinary tract infections (UTIs), particularly in young sexually active females.

•Catalase test result :- Staph saprophyticus is catalase-positive, meaning it produces the enzyme catalase. This enzyme allows the bacterium to convert hydrogen peroxide to water and oxygen. This characteristic is helpful in distinguishing Staphylococci from catalase-negative Streptococci and Enterococci.

•Blood agar test :- It forms white, raised, cohesive colonies about 1–2 mm in diameter , glossy, smooth, and convex. They have a buttery consistency and they may be white, cream, yellow, or orange (50%-65% of strains produce pigment), after overnight incubation, means it is not hemolytic on blood agar. coagulase-negative, facultative anaerobe that can grow by aerobic respiration or by fermentation.

•Staphylococcus saprophyticus can be definitively identified with other additional tests. First, perform a 2-hour PYR (L-pyrrolidonyl-ß-naphthylamide) broth hydrolysis test. If the result is negative, test the organism for production of urease, oxidase, alkaline phosphatase, and acid from D-trehalose to distinguish S. saprophyticus from other coagulase-negative, PYR-negative,

•MSA medium :- it dose not ferment the medium so the color of the media around the bacterial colony does not change to yellow, it appears pink. But take precaution some times Staphylococcus saprophyticus (coagulse negative Staphylococcus) may ferment mannitol, producing yellow halo around colonies in MSA thus resembling with S. aureus. So for confirmatory test use antibiotic Novobiosin.

•We can isolate Staphylococcus saprophyticus from mix culture of Staphylococcus species by using antibiotic Novobiosin, because it is resistant to this antibiotic. – when we mix the cultural medium with Novobiosin , all the Staph. strains will dye but S. sprophyticus will survive on that plate.

see photo for morphological apperance on plate