In: Chemistry
Two hFGF-1 mutants, A and B, are purified using a heparin-Sepharose column. Mutant A is eluted upon addition of 0.1M NaCl. Mutant B is eluted upon addition of 0.2 M NaCl. Which protein has a higher affinity for heparin? Is either protein expected to be 100% pure after this purification step?
A = 0.1 M
B = 0.2 M
higher affinity to NaCl will be the one with least concentration, since it elutes faster and "easier" on it
so
A elutes better on Nacl (ionic) solution than B
therefore
B has much more affinity toward the column,
The 100% separation is not likely to occur, since in chromatography or elution we have just partial separations, due to the coefficients of distributino in polar/ionic solvents