In: Biology

In an experiment in my laboratory, I used siRNA to decrease the amounts of a HDAC that removes the H3K9ac mark. What observations would I expect in terms of changes in gene expression?

HDAC is histone deacetylase enzyme. This enzyme is responsible for the removal of acetyl group from the amino acids of histone proteins.

Acetylation is the type of epigenetic regulation in which acetyl group is added to a specific amino acid of histone proteins. In general, acetylation tend to loosen the packing of chromatin and therefore increases gene expression. The loose packing of chromatin is responsible for increasing the accessibility of RNA polymerase towards the gene and therefore the rate of transcription increases.

Acetyl groups are added to histone protein by enzymes called as acetyltransferases are removed by deacetylases.

In the given question, we have decreased the amount of deacetylase. It means histone proteins will now remain acetylated. And when the proteins are acetylated, the rate of gene expression is more. So we can predict an increase in the rate of transcription.

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. A new vaccination is being used in a laboratory experiment to
investigate whether it is effective. There are 252 subjects in the
study. Is there sufficient evidence to determine if vaccination and
disease status are related?
Vaccination Status
Diseased Not
Diseased Total
Vaccinated
51)
54)
105)
Not Vaccinated
54)
73)
147)
Total
(125) (127) ( 252)
State the null and alternative hypothesis.
Find the value of the test statistic. Round your answer to three
decimal...

A new vaccination is being used in a laboratory experiment to
investigate whether it is effective. There are 246246 subjects in
the study. Is there sufficient evidence to determine if vaccination
and disease status are related?
Vaccination Status
Diseased
Not Diseased
Total
Vaccinated
60
55
115
Not Vaccinated
70
61
131
Total
130
116
246
home / study / math / statistics and probability / statistics
and probability questions and answers / a new vaccination is being
used in a...

A new vaccination is being used in a laboratory experiment to
investigate whether it is effective. There are 288 subjects in the
study. Is there sufficient evidence to determine if vaccination and
disease status are related?
Vaccination Status
Diseased
Not Diseased
Total
Vaccinated
81
45
126
Not Vaccinated
54
108
162
Total
135
153
288
Step 1 of 8: State the null and alternative hypothesis.
Step 2 of 8: Find the expected value for the number of subjects
who are...

A new vaccination is being used in a laboratory experiment to
investigate whether it is effective. There are 229 subjects in the
study. Is there sufficient evidence to determine if vaccination and
disease status are related?
Vaccination Status Diseased Not
Diseased Total
Vaccinated 68 71 139
Not Vaccinated 47 43 90
Total 115 114 229
Find the expected value for the number of subjects who are
vaccinated and are diseased. Round your answer to one decimal
place.
Find the expected...

A new vaccination is being used in a laboratory experiment to
investigate whether it is effective. There are 282 subjects in the
study. Is there sufficient evidence to determine if vaccination and
disease status are related?
Vaccination Status Diseased Not
Diseased Total
Vaccinated 54 124 178
Not Vaccinated 71 33 104
Total 125 157 282
Step 1 of 8:
State the null and alternative hypothesis.
Step 2 of 8:
Find the expected value for the number of subjects who are...

A new vaccination is being used in a laboratory experiment to
investigate whether it is effective. There are 339
subjects in the study. Is there sufficient evidence to determine if
vaccination and disease status are related?
Vaccination Status Diseased Not Diseased
Total
Vaccinated 66 152 218
Not Vaccinated 84 37 121
Total 150 189 339
Step 6 of 8 : Find the critical value of the test at the 0.005
level of significance. Round your answer to three decimal
places....

Hello for a laboratory experiment we used strain gauges to
measure the internal pressure of a coke can. Our instructor told us
one of the sources of error would be not aligning the gauges
perfectly. Why would this cause error in the experiment? Is there
any other sources of error as well.

describe a laboratory siphon experiment

I
used my catchement area to calculate my runoff using the national
method.
how will i go on calculating the volume needed for
storage?
which equations will i use ?
i want to have a storage tank that is pumped to the wetland.
how will i distribute the volume and calculate the sizing for
each.
Can you explain with an example if you can?

This is for my biochemistry lab, the experiment is dealing with
trypsin and BPTI. I need to make a graph: plot the absorbance
change per minute versus the BPTI concentration for each
cuvette.
here are my cuvettes and amount of BPTI added to each
1- 0uL BPTI added
2- 10 uL BPTI added
3-20 uL BPTI added
4-30 uL BPTI added
5- 40 uL BPTI added
6- 50 uL BPTI added.
Each cuvette has a different amount of water and...

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