Question

1. Fill in the rest of table 1: list the indicators (detection reagents) used to reveal the presence of the specific macromolecules:

Table 1

test (procedure)	Structure/Molecule detected:	Structure/Molecule found in:
Benedict‘s test		carbohydrate
Sudan III test
Biuret test	Peptide bonds

2. Fill in the rest of table 2: note the chemical explanation for the color changes observed in the macromolecule detection tests.

Table 2

Detention reagent	Explanation of detection
Benedict’s	Contains copper sulfate: copper binds to oxygen in the aldehyde or ketone group and the copper oxide that is formed transmits the orange/red color.
Sudan III
Biuret

Note: do not confuse the Benedict's Test for Non-Reducing Sugars with the

Answer 1

Test (procedure)	Structure/Molecule detected:	Structure/Molecule                  found	Explanation of detection
Benedict‘s test	simple sugar	Carbohydrate	Contains copper sulfate: copper binds to oxygen in the aldehyde or ketone group and the copper oxide that is formed transmits the orange/red color.
Sudan III test	Lipids	lipids	Sudan contains a non-polar red dye. it will make hydrophobic interactions with the hydrocarbon chain of lipids. Sudan III will change the color of the solution to reddish-orange
Biuret test	Peptide bonds	Protein	Copper(II) ions will form mauve-colored coordination complex in the presence of peptides in an alkaline soltion.