A Hfr strain possessing the markers His+ Met+ Tyr+ Strs was mated with a F– strain...

A Hfr strain possessing the markers His+ Met+ Tyr+ Strs was mated with a F– strain possessing the markers His– Met–Tyr– Strr.The time of entry for each marker is shown below.

Donor Marker Time (min)

his+ 21
met+ 12
tyr+ 46

a. Based on the results draw a map of the Hfr chromosome. Indicate the position of the F element, and the first marker to be transferred.

b. On what type of plates were cells grown to determine the above information?

Expert Solution

Ans:

a. If the marker takes less time, it is the first marker that enters into the recipient cell and cloest to F element. So, according to data, met+ is the first marker incorporated into recipient cell, followed by his+ and tyr+. So, the marker order is -F-met+-his+-tyr+. A drawing of map of the Hfr chromosome is given below-

b.

After 21 minutes, the cells grown on plate of minimal medium that lacks histidine and present of streptomycin (his- Strr)/ plate of minimal medium that lacks methionine and histidine and present of streptomycin (met- his- Strr).

After 12 minutes, the cells grown on plate of minimal medium that lacks methionine and present of streptomycin (met- Strr).

After 46 minutes, the cells grown on plate of minimal medium that lacks tyrosine and is streptomycin resistant (his- Strr)/ plate of minimal medium that lacks methionine, histidine and tyrosine and present of streptomycin (met- his- tyr- Strr).

gladiator answered 2 years ago

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