Determine the catalyst activity of trypsin.

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Expert Solution

Enzyme catalysis is the increase in the rate of a chemical reaction by the active site of a protein. The protein catalyst(enzyme) may be part of a multi-subunit complex, and/or may transiently or permanently associate with a Cofactor (e.g.adenosine triphosphate). Catalysis of biochemical reactions in the cell is vital due to the very low reaction rates of the uncatalysed reactions. A key driver of protein evolution is the optimization of such catalytic activities via protein dynamics.

The mechanism of enzyme catalysis is similar in principle to other types of chemical catalysis. By providing an alternative reaction route the enzyme reduces the energy required to reach the highest energy transition state of the reaction. The reduction of activation energy (Ea) increases the amount of reactant molecules that achieve a sufficient level of energy, such that they reach the activation energy and form the product. As with other catalysts, the enzyme is not consumed during the reaction (as a substrate is) but is recycled such that a single enzyme performs many rounds of catalysis

queen_honey_blossom answered 1 year ago

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