In: Biology
You have a phage stock with around 1.5x 107plaque-forming units per mL (PFU/mL). Design an a dilution scheme that could determine the titer of this phage stock. When deciding which dilutions to plate, plan for your scheme to generate a countable number of plaques if your stock has 1.5 x 106–1.5 x 107PFU/mL (10-fold off in either direction). For full credit, indicate which dilutions would be plated and how many PFU you would expect to see on those plates.