You have a phage stock with around 1.5x 107plaque-forming unitsper mL (PFU/mL). Design an a...

You have a phage stock with around 1.5x 107plaque-forming units per mL (PFU/mL). Design an a dilution scheme that could determine the titer of this phage stock. When deciding which dilutions to plate, plan for your scheme to generate a countable number of plaques if your stock has 1.5 x 106–1.5 x 107PFU/mL (10-fold off in either direction). For full credit, indicate which dilutions would be plated and how many PFU you would expect to see on those plates.

Solutions

Expert Solution

gladiator answered 2 years ago

Next > < Previous

Related Solutions

You have 0.5 ml of a stock solution of 1 mg/ml BSA. You need at least...

You have 0.5 ml of a stock solution of 1 mg/ml BSA. You need at least 200 ul of the solutions listed below. Calculate the volume of BSA needed and the volume of water needed to make the solutions. 25 ug/ml 50 ug/ml 75 ug/ml 100 ug/ml 150 ug/ml 200 ug/ml

Once you had obtained a highly concentrated stock of purified phage, how would you separate the...

Once you had obtained a highly concentrated stock of purified phage, how would you separate the recombinant phage DNA away from the phage head and tail proteins?

You are forming a portfolio with two stocks. The first stock has an expected return of...

You are forming a portfolio with two stocks. The first stock has an expected return of 15% and a standard deviation of 20%. The second stock has an expected return of 10% and a standard deviation of 15%. The two stocks have a correlation of 0.5. If you want to form the portfolio by investing $1000 in the first stock and $3000 in the second stock, what is the expected return and standard deviation of the portfolio? Lastly, use the...

In phage display using a phagemid vector derived from M13 filamentous phage, you need to transfect...

In phage display using a phagemid vector derived from M13 filamentous phage, you need to transfect a helper phage to propagate the phagemid vector. Explain why ?

You have 77.0 mL of a 0.500 M stock solution that must be diluted to 0.100...

You have 77.0 mL of a 0.500 M stock solution that must be diluted to 0.100 M. Assuming the volumes are additive, how much water should you add?

You have a stock of a DNA solution at 1.2mg/ml, the DNA fragment being 980bp in...

You have a stock of a DNA solution at 1.2mg/ml, the DNA fragment being 980bp in length. How do you prepare a dilution of this DNA that has a concentration of 0.5uM ?

1. You have a liquid sample that contains intact phage particles and ruptured bacterial cells. a....

1. You have a liquid sample that contains intact phage particles and ruptured bacterial cells. a. Where would you expect to find DNA in this sample? b. How can you treat this sample so that at the end of your work you will maximize the yield of phage genome and minimize the yield of potential interfering nucleic acids?

Match the microbe with the conditions described. Macrophages collect around the bacteria and fuse together forming...

Match the microbe with the conditions described. Macrophages collect around the bacteria and fuse together forming granulomas [ Choose ] Mycobacterium tuberculosis Helicobacter pylori Streptococcus pneumonia Enterobacteriacea Consists of closely related genera found in the large intestine [ Choose ] Mycobacterium tuberculosis Helicobacter pylori Streptococcus pneumonia Enterobacteriacea Is...

Imagine that you are forming a partnership with two other partners. All three of you have...

Imagine that you are forming a partnership with two other partners. All three of you have cash to invest in the business as well as skills to contribute. Two of your partners will provide services, along with investing cash. Are there any details you think should be included in the partnership agreement? What disadvantages should you be aware of when forming a partnership? How will you allocate income or loss? Will you also include a salary allowance for the partners...

(3) You have a 5 M sucrose stock solution and need 90 mL of the three...

(3) You have a 5 M sucrose stock solution and need 90 mL of the three following solutions for your experiment: 0.5 M, 0.05 M, and 0.005 M sucrose, respectively. How will you prepare the 3 sucrose solutions you need? You can use a diagram to explain your answer.

Subjects