Question

A Human Macrophage measures approximately 20 μm in Diameter. • Approximately how many Eyepiece Micrometer Units would a Macrophage span under your Olympus Microscope's 10X (Yellow) Lens, 40X (Blue) Lens and 100X (Oil Immersion) Lens?

Answer 1

Since the model No. or specifications such as reticle length, no.of divisions on it are not given. I'm taking some assumptions to explain this problem.

Let's assume we have 10mm reticle (eyepiece) with 100 divisions. Now, your reticle divison in this case would be 10mm/100 = 0.1mm.

Now for different objective lens this division or eyepiece unit will change.

For 10x, it is 0.1mm/10 = 0.01mm or 10 micrometers

For 40x, it is 0.1mm/40 = 0.0025mm or 2.5 micrometers

For 100x, it is 0.1mm/100 = 0.001mm or 1 micrometer.

Therefore, to answer the above question we will put the corresponding eyepiece unit for given objective lens and calculate the number of units.

So, for 10x(yellow), no. of eyepiece units = 20/10 = 2 units

for 40x(blue), no. of eyepiece units = 20/2.5 = 8 units

and for 100x(oil immersion), no. of units = 20/1 = 20 units.

Note, these no. will change accordingly if reticle length and no. of divisons. are different on your model of microscope.