Question

The allele "D" would be cleaved three times by EcoRI, resulting 4 fragments: 839, 262, and 215 bp, 151 bp. The allele with the “d” would be cleaved twice by EcoRI, resulting in 3 fragments: 753 bp, 512 bp, 202 bp.

a) If a plant has a genotype of DD, and the sample was digested with EcoRI. How many fragments do you have? And How many bands do you see on the gel electrophoresis for this sample?

b) If a plant has a genotype of Dd, and the sample was digested with EcoRI. How many fragments do you have? And how many bands do you see on the gel electrophoresis for this sample?

c) If a plant has a genotype of dd, and the sample was not digested by any enzyme. How many fragments do you have? And how many bands do you see on the gel electrophoresis for this sample?

d) If a plant has a genotype of Dd, and the sample was not digested by any enzyme. How many fragments do you have? And how many bands do you see on the gel electrophoresis for this sample?

Answer 1

Restriction enzymes cut the dsDNA at the specific site. Each restriction enzymes have unique restriction sites and always cut the DNA at that site and the number of fragments produced by restriction enzymes depends on the number of restriction sites present.

In the gel electrophoresis, fragments of DNA seprated on the gel based on the charge and molecular weight of DNA.

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