TLC or thin layer chromatography could be useful in the
determination of identity and purity of E-stilbene and stilbene
dibromide.
- It is useful in monitoring the progress of reaction, whether it
is proceeding or has gone to completion. The disappearance of one
spot of E-stilbene and the appearance of a different spot over time
from the reaction mixture on the TLC indicates that the E-stilbene
has been converted to stilbene dibromide. Further, when the
compounds are spotted together with a pure sample on a TLC plate,
the compound will show a single spot parallel to that of a pure
E-stilbene and stilbene dibromide.
The intermolecular forces could be helpful in predicting how
stilbene dibromide would move on a TLC plate relative to
E-stilbene
- Trans-stilbene will have relatively high Rf value in any given
condition and solvent as compared to the stilbene dibromide because
it is a non-polar molecule and so does not interact with the polar
molecules in the silica gel. Further, the bromination of E-Stilbene
results in greater polarity in the stilbene dibromide. Thus,
stilbene dibromide will bind to the polar stationary phase (low
Rf), while the non-polar E-Stilbene will travel well with the
non-polar mobile phase (high Rf).