Question

Define, differentiate, tell the cellular location and give an example of each of the two different types of ways a mammalian cell can generation ATP from ADP and Pi.

Answer 1

1) substrate level phosphorylation- a phosphate is transferred from an organic molecule directly at the ADP to make ATP.

2) oxidative phosphorylation- indirect synthesis of ATP that requires redox reactions involving O2 as a final electron acceptor and uses a electrochemical gradient.

substrate level phosphorylation	oxidative phosphorylation
Direct phosphorylation	indirect phosphorylation
Occurs in Krebs cycle ans glycolysis	occurs in ETC
4 ATP are produced	30 ATP are produced
Change in substrate redox potential is less	change in redox potential of substrate is more
Partial oxidation of substrate occurs	complete oxidation of substrate occurs

Location of substrate level phosphorylation is cytoplasm and mitochondrial matrix.

Location of oxidative phosphorylation is inner membrane of mitochondria.

Example of oxidative phosphorylation- oxidation of glucose into CO2 and water generate 28 ATP.

Example of substrate level phosphorylation- ATP synthesis, when PEP is converted into pyruvate by pyruvate kinase