Question

Use the information above to answer the following questions.

5’…TAC ACC GAT GGA TAA GTC…3’ DNA Coding strand

3’…ATG TGG CTA CCT ATT CAG…5’ DNA template strand

A. Write the sequences of the two double-stranded daughter DNAs which will be replicated from the sequence above. Indicate which sequences are directly from the parent. Show accurate direction of each sequence. 0.5 points

​​​​​​​B. Write the sequence of the mRNA strand that will be transcribed from the above DNA sequence. Show accurate direction of the sequence 0.5 points

C. Write the sequence of the tRNA strand carrying the anticodons which will hydrogen bond with the mRNA sequence complimentarily. Show accurate direction of the sequence 0.5 points

​​​​​​D. Using the mRNA codons table from your transcription/translation slides, predict and write out the amino acid sequence which will be translated from the mRNA transcript. Be sure to use the accurate open reading frame. Be careful you do not miss a step/nucleotide. 0.5 points.

​​​​​​​E. What will the amino acid sequence be If there was a point mutation in the original DNA sequence which changed the ‘second A’ to T in the DNA template strand? 0.5 points

F. ​​​​​​​Will the mutation in E affect the function of the peptide/protein? Why or why not? Note that it’s either Yes or No. You receive points for the reasoning only if you chose the right answer. 0.5 points.

ONLY answer E and F please!!

Answer 1

A. The given strands are

5’…TAC ACC GAT GGA TAA GTC…3’ DNA Coding strand

3' ... ATG TGG CTA CCT ATT CAG 5'

3’…ATG TGG CTA CCT ATT CAG…5’ DNA template strand

5'   TAC ACC GAT GGA TAA GTC 3'

The sequence in bold are parent strands and below them are their daughter strand.

2. For mRNA, the template strand is used. so

3’…ATG TGG CTA CCT ATT CAG…5’ DNA template strand

5' UAC ACC GAU GGA UAA GUC 3' mRNA strand.

3. The anticodon strand will be complementary to the mRNA

5' UAC ACC GAU GGA UAA GUC 3' mRNA strand.

3'  AUG UGG CUA CCU AUU CAG…5’ tRNA anticodon

4. For this mRNA, the amino acids will be

5' UAC ACC GAU GGA UAA GUC 3' mRNA strand.

3' Tyr Thr Asp Gly Stop 5'

5. 3’…ATG TGG CTT CCT ATT CAG…5’. The mutation from A to Twill form mRNA

  5' UAC ACC GAA GGA UAA GUC 3' mRNA which forms

3' Tyr Thr Glu Gly Stop 5'.

6. Yes, it will affect the peptide function. The mutation will affect the protein sequence as the 3rd amino acid is changed from aspartate to glutamine.