In: Chemistry
Terminology: 1.Thickness of internal coating of an analytical column (meaning, the purpose and the range of them (in um) 2.Carrier gas in GC and Eluent in LC ( general purpose, what is the general carrier media, gas or eluent, for GC amd LC 3. Isocratic and gradient program of HPLC 4. FID detector (full name, flow of the gases for flame, best use for what compounds)
1) Thickness determines the retention and elution temperature of each sample component. Thick films increase the time a compound stays on the stationary phase and thinner films reduce retention time. Compounds having high volatility require more residence time for better separation and should be analyzed on thicker films. The commonly used film thickness in gas chromatography columns ranges from 0.1 to 5.0µm.
2) Probably more than 90% of the present GC instruments run with helium as carrier gas. Some people use hydrogen or nitrogen. These gases serve to produce wind through the column to move our solutes forward. The solute molecules evaporate from the stationary phase surface, i.e., enter the open space of the capillary column, are hit by a carrier gas molecule and start traveling down the tube
4) A flame ionization detector (FID) is a scientific instrument that measures the concentration of organic species in a gas stream. It is frequently used as a detector in gas chromatography