For each primary antibody below, select what secondary antibody would have been used for fluorescent imaging...

For each primary antibody below, select what secondary antibody would have been used for fluorescent imaging in a green wavelength:

a) Mouse anti-GFAP: goat anti-mouse Alexa488 or goat anti-rabbit Alexa488 b) Rabbit anti-NF200: goat anti-mouse Alexa488 or goat anti-rabbit Alexa488 c) Rabbit anti-PSD95: goat anti-mouse Alexa488 or goat anti-rabbit Alexa488

Expert Solution

a) For primary antibody Mouse anti-GFP, the secondary antibody should be the goat anti-mouse Alex 488. This is because the host for the primary antibody is the mouse. The secondary antibody's main use is in detection to achieve this it should be specific to the primary antibody.The secondary antibody is always against the host in which the primary antibody is raised. It is the Fc region of the primary antibody to which secondary antibody binds. The secondary antibody goat anti-mouse Alexa 488 is raised in goat against the mouse. Alexa-488 is a green fluorescent dye which is conjugated with secondary antibody. The dye has absorption maxima at 496 mm and emission around 519 mm and gives green color fluorescence (green wavelength 560-520 nm).

b) For primary rabbit anti-NF200, the secondary antibody should be goat anti-rabbit Alexa 488. The secondary antibody goat anti-mouse Alexa 488 can not be used as a secondary antibody it is raised in the mouse.

c) For primary antibody rabbit anti-PSD95, the secondary antibody should be the goat anti-rabbit Alexa 488. The antibody goat anti-mouse Alexa 488 can not be used as secondary because it is raised in the mouse, not in the rabbit.

gladiator answered 2 years ago

What laboratory tests are used to assess disorders of primary hemostasis? Secondary hemostasis?

what would be an example of primary, secondary, and tertiary prevention for type 2 diabetes ?

What are emotions? What is the difference between primary and secondary emotions? What are the primary...

What are emotions? What is the difference between primary and secondary emotions? What are the primary and secondary emotions? What is the developmental timecourse of primary and secondary emotions?

List a primary, secondary, and tertiary intervention for breast cancer, what would you recommend as a...

List a primary, secondary, and tertiary intervention for breast cancer, what would you recommend as a screening policy?

What is the difference between primary and secondary apnea?

What is the difference between primary, secondary and tertiary research? When is each kind of research...

What is the difference between primary, secondary and tertiary research? When is each kind of research best used? What kind of research should you generally be using in your business courses?

1 What is the difference between primary, secondary, and tertiary sources? Give an example of each....

1 What is the difference between primary, secondary, and tertiary sources? Give an example of each. 2 What are the key elements of a research work plan? 3 What is a desktop search tool and how can it help you? 4 What is the effect of using quotation marks around search terms in web or database searches? What happens if you do not use quotation marks? 5 Explain the three criteria for evaluating whether a source is credible. 6 Name...

Compare and contrast the role of the primary and secondary stock markets. What function do each...

Compare and contrast the role of the primary and secondary stock markets. What function do each of these markets satisfy?

what stakeholders would you include in an accreditation? how would you ensure your primary, secondary. tertiary...

what stakeholders would you include in an accreditation? how would you ensure your primary, secondary. tertiary stakeholders informed?

1) What properties would you consider important for designing a fluorescent probe that could be used...

1) What properties would you consider important for designing a fluorescent probe that could be used in vivo to study an enzyme activity found in blood vessels? 2) What is the difference between an epitope tag and a fusion protein? Describe how you might image dynamics of centrioles in live cells during cell division assuming you use a genetic approach involving creating a fusion protein. Use diagrams and words to explain what proteins you would use to do this and...

Question