Question

In: Biology

a. You are conducting a lab experiment on the promoter regions of genes. In one experiment...

a. You are conducting a lab experiment on the promoter regions of genes. In one experiment you apply heat to two sections of DNA that you believe could be promoter regions. One region unwinds at 80 degrees C and the other unwinds at 60 degrees C. Which of the two strands contain the promoter? Explain.

b. Apply your knowledge of genetics to explain what would happen in a nucleus if histones were negatively charged.

Solutions

Expert Solution

a. According to the research, it has proved that -10 region of promoter shows temperature sensitive activity. Thus it starts to unwind the DNA from a particular temperature but rising temperature affects its operation.

Thus, the section of DNA that unwinds at 60 degrees C, contains promoter.

b. With the help of histones, chromosomal DNA is packed inside microscopic nuclei. Generally, histones are positively charged proteins that bind strongly to negatively charged DNA to form structures called nucleosomes. Without DNA packaging, a cell cannot support vast amounts of DNA that are contained within it, thus it is an essential mechanism in living cells. DNA packaging is also important because it means that such abundant DNA will fit perfectly in a cell that is several times smaller. Moreover, nucleosomes shield the genome from harmful damaging agents of DNA and provide a mesh to store a variety of epigenetic signals.
So, if histones were negatively charged, it will not able to wind up the huge DNA structure, as DNA is also negatively charged. Thus it becomes impossible for DNA to fit into the tiny nucleus. Not only this, but DNA also becomes prone to damage by harmful agents.

gladiator answered 2 hours ago
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