In: Biology
Shown below is a list of molecules that function in fruit fly development and a list of distribution patterns. Match the molecules to their normal distribution in fruit fly embryos.
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(Put each letter to each Answer)
Two-dimensional gel electrophoresis is used to characterize the physical characteristics and expression levels of:
| A. |
proteins |
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| B. |
lipids |
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| C. |
microtubules |
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| D. |
DNA polymorphisms |
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| E. |
mRNA |
The end product of a multistep process that includes 1) digestion of genomic DNA by restriction enzymes, 2) ligation of this DNA into vectors, 3) transformation of bacteria with the recombinant DNA molecules, and 4) isolation of individual bacterial clones that carry this recombinant DNA.
| A. |
Genomic library |
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| B. |
CD library |
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| C. |
cDNA library |
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| D. |
Gene expression |
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| E. |
RNA library |
Long stretches of uninterrupted codons in genomic DNA are known as:
| A. |
open reading frames |
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| B. |
amino acids |
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| C. |
stop codons |
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| D. |
translocations |
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| E. |
point mutations |
1. Distributed uniformly in the oocyte/embryo - hunchback mRNA.
Highest concentration in anterior end of the embryo - hunchback proteinp
highest concentration in posterior end of embryo - nanos mRNA
Highest concentration in the ventral part of the embryo - spaetzle protein
2. Two-dimensional gel electrophoresis is used to characterize the physical characteristics and expression levels of:
protein
3. The end product of a multistep process that includes 1) digestion of genomic DNA by restriction enzymes, 2) ligation of this DNA into vectors, 3) transformation of bacteria with the recombinant DNA molecules, and 4) isolation of individual bacterial clones that carry this recombinant DNA.
ans. genomic library
4. Long stretches of uninterrupted codons in genomic DNA are known as:
open reading frame
ORF or Open Reading Frame is a length of a genome sequence that is a series of amino acid specifying triplet codons where the targeted genes are identified for the prediction analysis. The long stretches of sequences starts from the start codon and are uninterrupted by a stop codon, and usually consist of approximately 100 codons.Any particular stretch of DNA will have six reading frames that could potentially code for a functional protein.