Question

In: Biology

Shown below is a list of molecules that function in fruit fly development and a list...

Shown below is a list of molecules that function in fruit fly development and a list of distribution patterns. Match the molecules to their normal distribution in fruit fly embryos.

      -       A.       B.       C.       D.   

Distributed uniformly in the oocyte/embryo

      -       A.       B.       C.       D.   

Highest concentration in anterior end of the embryo

      -       A.       B.       C.       D.   

Highest concentration in the posterior end of the embyo

      -       A.       B.       C.       D.   

Highest concentration in the ventral part of the embryo

A.

hunchback mRNA

B.

hunchback protein

C.

nanos mRNA

D.

Spaetzle protein

(Put each letter to each Answer)

Two-dimensional gel electrophoresis is used to characterize the physical characteristics and expression levels of:

A.

proteins

B.

lipids

C.

microtubules

D.

DNA polymorphisms

E.

mRNA

The end product of a multistep process that includes 1) digestion of genomic DNA by restriction enzymes, 2) ligation of this DNA into vectors, 3) transformation of bacteria with the recombinant DNA molecules, and 4) isolation of individual bacterial clones that carry this recombinant DNA.

A.

Genomic library

B.

CD library

C.

cDNA library

D.

Gene expression

E.

RNA library

Long stretches of uninterrupted codons in genomic DNA are known as:

A.

open reading frames

B.

amino acids

C.

stop codons

D.

translocations

E.

point mutations

Solutions

Expert Solution

1. Distributed uniformly in the oocyte/embryo - hunchback mRNA.

Highest concentration in anterior end of the embryo - hunchback proteinp

highest concentration in posterior end of embryo - nanos mRNA

Highest concentration in the ventral part of the embryo - spaetzle protein

2. Two-dimensional gel electrophoresis is used to characterize the physical characteristics and expression levels of:

protein

3. The end product of a multistep process that includes 1) digestion of genomic DNA by restriction enzymes, 2) ligation of this DNA into vectors, 3) transformation of bacteria with the recombinant DNA molecules, and 4) isolation of individual bacterial clones that carry this recombinant DNA.

ans. genomic library

4. Long stretches of uninterrupted codons in genomic DNA are known as:

open reading frame

ORF or Open Reading Frame is a length of a genome sequence that is a series of amino acid specifying triplet codons where the targeted genes are identified for the prediction analysis. The long stretches of sequences starts from the start codon and are uninterrupted by a stop codon, and usually consist of approximately 100 codons.Any particular stretch of DNA will have six reading frames that could potentially code for a functional protein.


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