Question

Which of the following is not a role nor a property of the mediator?

• serves as bridge between gene-specific transcription co-activators and RNA pol II

• provides a scaffold for the assembly of the preinitiation complex

• acts as a regulator for the basal expression of RNA polI-dependent genes

• binds to response elements to recruit transcription factors and RNA pol II

Answer 1

Which of the following is not a role nor a property of the mediator?

Ans) binds to response elements to recruit transcription factors and RNA pol II

Explanation- Mediator of RNA polymerase II transcription does not directly bind DNA ( i.e. response elements) but interacts with chromatin through intermediates: transcription factors at enhancer regions and preinitiation complex (PIC) components at core promoters

Others than this all stated roles are correct

Mediator complexes interact with transcription factors and RNA polymerase II. The main function of mediator complexes is to transmit signals from the transcription factors to the polymerase.

Mediator has been shown to make contacts with the polymerase in the transcription preinitiation complex

Mediator interacts with the pre-initiation complex, composed of RNA Polymerase II and general transcription factors TFIIB, TFIID, TFIIE, TFIIF, and TFIIH to stabilize and initiate transcription.

Mediator helps regulate gene-specific transcription and enhances basal transcription.

Ps- An upvote would be highly appreciated

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