Question

What is the purpose of matching cuvettes? Explain how it is done. ( please and thank you)

Answer 1

Matching Cuvettes - A pair of cuvettes which are made of same material and has the same thickness (optical length) are known as matching cuvettes

This concept was started at a time when spectrophotometer cells were not made very well and most absorbtion instruments were single beam (no ability to automatically adjust for the blank). But now high quality glass and Quartz cuvettes have come and advanced spectrometers with double beam technology were designed and matching cuvettes were become less important.

Matching quvettes must be made of same material and have same optical length

When filled with same solvent, spectra of the matching quvettes must be identical

Matching quvettes is useful in the measurements of absorption spectra of compounds in the wavelenght range where the solvent or the cuvette itself absorb the light or when the subject of investigation is the difference in absorption of two samples