Question

In: Biology

Describe the features of the gene organization and the gene structure between bacteria and human. How...

Describe the features of the gene organization and the gene structure between bacteria and human. How do these differences of genomic features provide evolutionary adaptations to prokaryotic and eukaryotic organismal functions.

Why are multiple specific bacterial tester strains used in the Ames test? Illustrate with a diagram, how can the toxic and mutagenic effects be distinguished from the anticipated results?

Solutions

Expert Solution

1. Genome of bacteria are arranged in the cell in a confined space, the nucleoid. This arrangement is dynamic allowing for DNA transactions such as replication, transcription and repair to occur at appropriate times.The genome of human is organized into 22 paired chromosomes, termed autosomes, plus the 23rd pair of sex chromosomes (XX) in the female, and (XY) in the male. These are all large linear DNA molecules contained within the cell nucleus.

Bacteria are typically haploid, usually having a single circular chromosome found in the nucleoid. Eukaryotes are diploid; DNA is organized into multiple linear chromosomes found in the nucleus.

Supercoiling and DNA packaging using DNA binding proteins allows lengthy molecules to fit inside a cell. Eukaryotes use histone proteins, and bacteria use different proteins with similar function.

Bacterial and human genomes both contain noncoding DNA, the function of which is not well understood. Some noncoding DNA appears to participate in the formation of small noncoding RNA molecules that influence gene expression; some appears to play a role in maintaining chromosomal structure and in DNA packaging.

Extrachromosomal DNA in humans includes the chromosomes found within organelles of prokaryotic origin (mitochondria and chloroplasts) that evolved by endosymbiosis.

Extrachromosomal DNA in bacteria is commonly maintained as plasmids that encode a few nonessential genes that may be helpful under specific conditions. Plasmids can be spread through a bacterial community by horizontal gene transfer.

2. Bacterial genomes increase in size by aggregating adaptive gene modules when exposed to new selective pressures, whereas human genomes increase in size by accumulating large amounts of non-functional DNA when exposed to drift.

3. The Ames test is a widely employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. More formally, it is a biological assay to assess the mutagenic potential of chemical compounds.

Some compounds are quite specific, causing reversions in just one or two strains. The tester strains also carry mutations in the genes responsible for lipopolysaccharide synthesis, making the cell wall of the bacteria more permeable, and in the excision repair system to make the test more sensitive.

Ames test uses several strains of bacteria (Salmonella, E.coli) that carry mutation. Eg A particular strain of Salmonella Typhimurium carry mutation in gene that encodes histidine. So it is an auxotrophic mutant which loss the ability to synthesize histidine (an amino acid) utilizing the ingredients of culture media. Those strains are known as His- and require histidine in growth media.

Culturing His- salmonella is in a media containing certain chemicals, causes mutation in histidine encoding gene, such that they regain the ability to synthesize histidine (His+) This is the reverse mutation. Such chemicals responsible to revert the mutation is actually a mutagen. So, this Ames test is used to test mutagenic ability of varieties of chemicals.


Related Solutions

Describe the features of the gene organization and the gene structure between bacteria and human. How...
Describe the features of the gene organization and the gene structure between bacteria and human. How do these differences of genomic features provide evolutionary adaptations to prokaryotic and eukaryotic organismal functions.
In relation to gene organization and structure, explain the difference between single genes, gene families and...
In relation to gene organization and structure, explain the difference between single genes, gene families and tandemly repeated genes
Choose an example of an organization with a mechanistic structure. Describe the features that lead you...
Choose an example of an organization with a mechanistic structure. Describe the features that lead you to believe the firm is mechanistic. Comment on whether you think this is a good fit based on the firm's overall strategy, capabilities, and industry.
describe the structure of a bacteria flagella and how is the bacteria flagella different from a...
describe the structure of a bacteria flagella and how is the bacteria flagella different from a eukaryotic one? Explain the following terms (in relation to bacteria flagella) – Peritrichous, Monotrichous, Lophotrichous, and Amphitrichous What are axial filaments – what microbes have axial filaments? Distinguish between Fimbriae and Pili List 3 functions of Pili
Please answer all Describe transcription and translation in bacteria. How is gene regulation in bacteria achieved?...
Please answer all Describe transcription and translation in bacteria. How is gene regulation in bacteria achieved? What is the two component system of regulation? Give some examples of negative and positive regulation. How do the sensor and response proteins get activated?
Describe the key features of each structure including function, organization, cellular location, and some of the most important molecules that characterize the structure.
Describe the key features of each structure including function, organization, cellular location, and some of the most important molecules that characterize the structure.a. Ribosomeb. Nucleolusc. Nuclear Pore Complexd. Endoplasmic ReticulumWhat type of microscopy could you use to “see” the structure? Would your method allow a direct or indirect visualization? Explain your choice based on structure size and the resolution needed to detect the structure.These are the tools that we covered: transmission electron microscopy (TEM), electron microscopy, fluorescence microscopy, immunofluorescence microscopy....
Describe the accounting and finance structure in an organization.
Describe the accounting and finance structure in an organization.
Describe Cartier current Organization structure ?
Describe Cartier current Organization structure ?
Membranes are important structural features of cells. Describe how membrane structure is related to the transport...
Membranes are important structural features of cells. Describe how membrane structure is related to the transport of materials across the membrane. Describe the role of membranes in the synthesis of ATP in either cellular respiration or photosynthesis.
4. Describe the features of the stomach and small intestine that are involved in the human...
4. Describe the features of the stomach and small intestine that are involved in the human digestion process.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT