In: Chemistry
A monoprotic acid with a Ka of 7.79E10-5 has a partition coefficient of 3.4 (favoring octanol) when distributed between water and octanol. Find the formal concentration of the acid in each phase when 100 mL of 0.10 M aqueous acid is extracted with 31 mL of octanol at (a) pH 4.00 and (b) pH 9.00.
Ka of acid = 7.79x10-5
Partition coefficient, KD = 3.4
Volume of octanol = 31 mL
Milimoles of acid = 100 * 0.10 = 10 mmoles
(a) at pH 4.00
[H +] = 10-4 M
Distribution ratio, D = KD * [H+] / [H+] + Ka
D = 3.4 x 10-4 / 10-4 + 7.79x10-5
D = 3.4 x 10-4 / 1.779 x 10-4
D = 1.91
Fraction of acid remaining in aqueous phase -
Q(aq) = 100 / 1.91*31 + 100
Q(aq) = 0.62
Moles of acid in aqueous phase = 0.62 * 10
= 6.2
Concentration of acid in aqueous phase = 6.2 / 100 = 0.062 M
Moles of acid in organic phase = 10 - 6.2 = 3.8
Concentration of acid in organic phase = 3.8 / 31 = 0.122 M
(b) at pH 9.0
[H +] = 10-9 M
Distribution ratio, D = KD * [H+] / [H+] + Ka
D = 3.4 x 10-9 / 10-9 + 7.79x10-5
D = 3.4 x 10-9 / 7.7901 x 10-5
D = 4.36x10-5
Fraction of acid remaining in aqueous phase -
Q(aq) = 100 / 4.36x10-5 *31 + 100
Q(aq) = 0.99
Moles of acid in aqueous phase = 0.99 * 10
= 9.9
Concentration of acid in aqueous phase = 9.9 / 100 = 0.099 M
Moles of acid in organic phase = 10 - 9.9 = 0.1
Concentration of acid in organic phase = 0.1 / 31 = 0.0032 M