Question

Case: Leo is a histology student taking histotechniques laboratory course. He has obtained 5 different organs during a rat scarification and wants to make histologic slides out of them. His supervisor asked him to show different tissue types and tissue components using specific staining methods (given below).

Please describe in 2 pages (minimum 800 words) which tissue processing (all the steps from histotechniques lecture notes) and specific staining methods (find in reliable websites, books and articles) Leo should choose and why he needs to use these specific methods to be able to show required structures.

Obtained Organs: Required tissue structure Tissue processing method and Specific staining

1. Liver Glycogen inclusions …

2. Femur Bone tissue …

3. Skin Collagen bundles …

4. Lymph node Reticular fibers …

5. Spinal cord Nissl bodies

Case: Leo is a histology student taking histotechniques laboratory course. He has obtained 5 different organs during a rat scarification and wants to make histologic slides out of them. His supervisor asked him to show different tissue types and tissue components using specific staining methods (given below). Please describe in 2 pages (minimum 800 words) which tissue processing (all the steps from histotechniques lecture notes) and specific staining methods (find in reliable websites, books and articles) Leo should choose and why he needs to use these specific methods to be able to show required structures. Obtained Organs: Required tissue structure Tissue processing method and Specific staining 1. Liver Glycogen inclusions … 2. Femur Bone tissue … 3. Skin Collagen bundles … 4. Lymph node Reticular fibers … 5. Spinal cord Nissl bodies

Answer 1

1. Glycogen is that part of the liver that stores the glucose.First the liver tissue is treated with hot alkali and then homogenated to get the glycogen inclusions.The periodic acid-schiff stain is used to identify glycogen.Glycogen is removed by diastase digestion,due to that small amount of nn digested material can be also present.Try to write more about glycogen and the stain.

2. Femur bone is also known as thighbone.Tissues can be obtained from bone fragments or bone pieces and it is stained with tetrazolium salt.The samples can be stained with 2,3,5-triphenyl-2H-tetrazolium chloride at different times.

3. Collagen is a strong protein and is responsible for the skin elasticity.It can obtained from ligaments and tendon.It is stained using hemotoxylin and eosin.

4.Reticular fibres are composed of collagen and it forms a framwork around nerve fibres,lymph nodes etc...It is stained using trichrome-masson.