In genetic modifications, DNA inserted can come from _____ . only the same species as the...

In genetic modifications, DNA inserted can come from _____ .

only the same species as the vector

only the same plasmid as the vector

only the same phylum as the plasmid vector

any organism containing the DNA of interest

only the same species as the host

Solutions

Expert Solution

Answer. 4

The 1st recombinant DNA molecule was made by Boyer & Cohen in 1973.

Any DNA molecule from any orgnaism or artificially made DNA can be inserted to make a recombinant DNA molecule, as DNA is afterall DNA.

Restriction enzymes and ligases help to cleave of the gene of interest and ligate with any DNA sequence.

Class II restriction enzymes are of value to genetic engineering and recognize a palindromic sequence for cleavage. They can create either sticky ends or blunt ends. Ligation of sticky ends is easier than that of blunt ends.

Following steps are involved in creating a recombinant DNA molecule and eventually a recombinant protein -

Identification of gene of interest.
Isolation of genes.
Insertion of the isolated gene into a vector.
Transformation into a host.
Identification of recombinant clone.
Growth of the recombinant clone.
Isolation and purification of recombinant protein.
Characterization of the recombinant protein.

gladiator answered 1 year ago

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