In: Biology
2. Let’s assume that you do find movement utilizing cytoplasmic streaming. However, a new question is now brought up. Is this movement dependent on cytoskeletal elements and motor proteins and if so, which ones? Describe how you could test to see which cytoskeletal elements/motor proteins were responsible for the movement of your protein. What would your data look like? Note you can’t knockout any motor proteins or cytoskeletal elements. They are essential to life; if you knock them out, the cell dies within a day or so. You have to find another method. Be creative.
The proteins involved the cytoplasmic streaming are the microfilament protein Actin & the motor protein Myosin. This occurs in a fashion similar to the sliding filament theory of muscular contraction where myosin proteins slide on the actin filaments with the help of energy from ATP.
Experiment to test which cytoskeletal elements/motor proteins are involved:
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