All bacteria are able to use citrate for energy or as a carbon source. That being...

All bacteria are able to use citrate for energy or as a carbon source. That being said, only some bacteria can grow on citrate media. Explain why bacteria that can use citrate for energy and carbon may not be able to grow on this media.

Expert Solution

simmons citrate agar test the ability of organism to utilise citrate as a carbon source . if the medium turn blue , then organism is citrate positive. if there is no colour change, the organism is citrate negative. citrate agar is used to test an organism ability to utilise citrate as a source of energy that does not require the to use the citrate as a sole carbon source. the citrate negative bacteria can also grow on this medium .

some organism are capable of growth on citrate and do not produce a color citrate test. simmons citrate agar test the ability of organism to utilise citrate as a carbon source. simmons citrate agar contain sodium citrate as the sole of source of carbon, ammonium dihydrogen phosphate as the sole source of nitrogen, other nutrients and pH indicator bromthymol blue . klebsiella pneumoniae and proteus mirabilis are example of citrate positive organism. escherichia coli and shigella dysentries are citrate negative. the purpose is to see if the microbe can be use the compound citrate as its soul source of carbon and energy for growth. abundant growth on the slant and a change from green into blue in the medium indicate a positive test for growing using citrate.

gladiator answered 8 hours ago

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