Question

describe experiment that can be used to differentiate motor proteins moving in different directions and describe a method to determine and measure the average steps the motor would take.

Answer 1

motor proteins or also said to be hall molecular motors. it has the capability to transport the loads only if it is attached with a ATP ( adenosine triphosphate) example for motor proteins for kinesin and dyenin. which is essential for many important cell functionality.

the directionality of the motor proteins and its motion towards the cell components can be determined related to the structural position of the water motor protein. For kinesin- the locations for the motor domain are N-terminal, C terminal and in middle of the molecule. the motility of the protein can be tracked by using microscopic techniques like DIC ( differential interference contrast) and fluorescence microscopy.

Atomic force microscopy can be used as a force detection technique that can also modified to measure the motility.

along with that force spectroscopy techniques such as optical trapping magnetic tweezers used for assessing the motor protein stall force.

mathematical models also used to investigate and predict the force and motility of kinesin and dyenin in addition to the experimental techniques.