Question
In: Biology
Why is DNA replication called a semi-conservative mechanism? What is the difference between the leading and...
Why is DNA replication called a semi-conservative mechanism?
What is the difference between the leading and the hesitant (lagging) thread in DNA replication, and why must a leading and a lagging thread be formed?
What are the origin site, topoisomerase/gyrase, and primer
respectively
connection with the replication?
What function does helicase (DnaB) and DNA polymerase III
have
replication?
What do DNA polymerase I and ligase contribute to?
Solutions
Expert Solution
1. I DNA replication mechanism both strands of DNA replicate and from two different daughter strand and in new DNA molecule one is a parent and the other is daughter strand.
2. The template strands form a leading strand in which DNA replication is continuous. The other strand is lagging strands in which DNA replication is non- continuous.
The lagging and leading strand are formed due to single DNA polymerase and due to the inability to do replication on the 5' to 3' direction.
3. The origin site the place where the replication starts.
Topoisomerase release the pressure in the double helix during unwinding OF DNA helix.
The primer is a single-stranded RNA that binds the template strand and helps in replication by providing a site for elongation by DNA polymerase.
4. The helicase unwinds the DNA helix because replication only occurs when DNA opens.
The DNA polymerase III burns to the open strdns of DNA and starts the elongation process of replication.
5. DNA Polymerase I is used in the repairing process of DNA.
ligase help in Ligation of repaired strands and in the ligation of Okazaki fragments in the lagging strand.
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