What does “semi-discontinuous” DNA replication mean? Explain in
words how the lagging DNA strand is replicated in bacteria. What
specific enzymatic activities does DNA polymerase I have? Name each
activity and explain its role in DNA replication. If DNA polymerase
I lacked its 3’-5’ exonuclease activity, what specific consequences
with respect to DNA replication would be observed? What
consequences would be observed if, instead of DNA pol I, DNA
polymerase III lacked its 3’-5’ exonuclease activity? Explain your
answers.
1.Describe the synthesis of the leading strand for DNA replication.
2. How the lagging strand differ from the leading
strand?
3. what is semiconservative replication?
9.) During the process of DNA replication, both DNA polymerase
and RNA polymerase are involved in eventually creating the new
daughter strand of DNA. T or F
10.) Replication begins at the origin of replication or ORI and
transcription begins at the first codon for methionine. T or F
11.) The process of mapping, sequencing and analysis of proteins
is called genomics. T or F
12.) The heavy chain constant domains of a given immunoglobulin
molecule define its class. T...
What would happen if we use Polymerase I instead of Polymerase
III for DNA replication in a PCR? If we used an enzyme with low
fidelity, how would the DNA product compare to the original
template?
1. Why does E. coli need both DNA polymerase III and DNA
polymerase I?
a. The DNA replication is bidirectional; one polymerase is used
for each direction.
b. Each polymerase is specific for only one strand of DNA. DNA
polymerase III acts only on the leading strand, and DNA polymerase
I acts only on the lagging strand.
c. Only DNA polymerase I has proofreading ability.
d. DNA polymerase III lacks the 5' → 3' exonuclease activity
needed to remove RNA...
Describe the synthesis of an Okazaki fragment on the lagging
strand in a replication fork. Include names of enzymes and the
proper 5’ and 3’ ends of DNA strands.