1. Which step is the most important in order for the Gram stain to be differential?...

1. Which step is the most important in order for the Gram stain to be differential? and Why?

Expert Solution

gladiator answered 2 months ago

What is the purpose of the iodine in the Gram stain procedure? If this step was...

What is the purpose of the iodine in the Gram stain procedure? If this step was left out, what color would Gram negative and Gram positive cells appear after completion of the stain?

"The most widely used stain in microbiology is the Gram stain. Based on differences in the...

"The most widely used stain in microbiology is the Gram stain. Based on differences in the structure of the bacterial cell wall, the Gram stain divides bacteria into two major groups: Gram-positive and Gram-negative. Gram-positive cells have a thick layer of peptidoglycan, a polymer made of amino acids and sugars, in the cell wall. Crystal violet binds to peptidoglycan, rendering the cell purple. Gram-negative cells also have peptidoglycan and initially also stain purple. Since the peptidoglycan layer is much thinner,...

The Gram stain differentiates Gram + bacteria from Gram – bacteria because of which of the...

The Gram stain differentiates Gram + bacteria from Gram – bacteria because of which of the following? Group of answer choices presence of an outer membrane in one group and absence in the other presence of teichoic acid in one group and absence in the other presence of ether-linked lipids in one group and absence in the other presence of peptidoglycan in one group and absence in the other difference in thickness of peptidoglycan layer between the two groups

1. In gram stain if at the end of the procedure both the Gram-positive and Gramnegative...

1. In gram stain if at the end of the procedure both the Gram-positive and Gramnegative bacteria appear red what was the most common Technical error 2. In Gram stain you stop the procedure atter decolonization how would the Gram-negative bacteria appear? How would the Gram-positive

1.) You are asked to prepare a gram stain from a mixture of gram positive and...

1.) You are asked to prepare a gram stain from a mixture of gram positive and gram negative bacteria during lab. What would happen if you remembered all steps.... except to decolorize – what color would the Gram (+) and Gram (-) be? except to add safranin – what color would the Gram (+) and Gram (-) be? 2.) The organism Yersinia pestis causes the plague, which caused a pandemic in Europe. What test reults would you expect to observe...

1. Can iodine be added before the primary stain in a Gram stain? Explain. If iodine...

1. Can iodine be added before the primary stain in a Gram stain? Explain. If iodine was added before the primary stain, what would be the final color of gram positive and gram negative cells in the stain and why? 2. Since you can’t identify bacteria from a Gram stain, why might a physician perform a Gram stain on a sample before prescribing an antibiotic? 3. The cell envelope of Mycobacterium sp. is unlike the envelope of other bacteria. While...

Is the culture medium: Phenyl ethyl Alcohol ( PEA) Selective Differential Bacteria Gram stain Growth Changes...

Is the culture medium: Phenyl ethyl Alcohol ( PEA) Selective Differential Bacteria Gram stain Growth Changes in the culture medium or bacteria Escherichia coli Negative no Pseudomonas fluorescens negative no Enterobacter aerogenes negative no Alcaligenes faecalis Negative no Micrococcus luteus Negative yes No changes Staphylococcus epidermidis positive yes No changes Enterococcus faecalis positive Yes No changes Bacillus cereus positive Yes No changes Only 1 Only 2 Both 1 and 2 Neither 1 or 2 Syprogyra is ________. A eukaryotic organism...

For this discussion question, in your own words, describe why the Gram stain is so important...

For this discussion question, in your own words, describe why the Gram stain is so important to all of microbiology, how it is performed, how it works, and compare the cell compositions it contrasts between Gram positive and Gram negative cells. Include why it is of such particular importance for medical microbiology. Also, giving two specific medically-significant microbe examples and descriptions for each group.

A.)There are four important steps in a Gram stain. List the specific stains/chemicals used in each...

A.)There are four important steps in a Gram stain. List the specific stains/chemicals used in each step, and the color of both Gram positive and Gram negative bacteria after each step. B.)Describe 2 reasons why heat fixing a specimen before viewing under a microscope is important. C.)Describe how the design of the swan - necked flasks in Pasteur's classic experiment helped disprove spontaneous generation. What were the results of the experiment? How did it counter the "vital force" argument believed...

A Gram stain is made of a fixed smear from a culture of Escherichia coli. Which...

A Gram stain is made of a fixed smear from a culture of Escherichia coli. Which of the following microscopes would be most suitable for observing this bacterium? Bright field microscope Phase-contrast microscope Transmission electron microscope Dark-field microscope Scanning electron microscope True or False. Molecular phylogenetics aims to classify organisms based on observable traits, such as morphological, biochemical and physical characteristics. Which of the following is NOT true concerning the plasma membrane of a cell? A. it is...

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