Question

In: Biology

You want to characterize the function of the Cellulose synthase-like C, CslC, gene that has been...

You want to characterize the function of the Cellulose synthase-like C, CslC, gene that has been reported to be involved in the biosynthesis of xyloglucan in higher plants. Your lab specializes in working with the moss, Physcomitrella patens. The CslC gene has been identified in this moss through genome sequence analysis, but it has yet to be reported if the moss gene really makes xyloglucan. It has also been reported in higher plants, that the CslC protein complex has been localized to the golgi apparatus. You are tasked with finding out what this gene does in moss and also localizing it’s respective protein complex within the cell. Outline how you would go about this task and list each molecular method that would be used

Solutions

Expert Solution

According to the information, it is desired to know the function of a gene in the moss. Secondarily, the objective of this experiment is to understand the localization of the protein complex generated from this gene. These two tasks can be performed as below:

Experiment 1: To know the function of gene

In vitro synthesis of knock-out variety of the moss can be developed by using recombinant DNA technology. These recombinant mosses can be used to understand the failure in a specific biological function as compared to the normal one. The function lacking in the recombinant moss will be the one controlled by the gene of interest.

Experiment 2: To know the localization of the protein comlex

In vitro tagging of the DNA sequence for this gene can be performed. This DNA sequence will then be sequenced and amplified by PCR. The gene can be subjected to cell-free translation and then tagged with appropriate fluorescent tags. This tagged protein can be inserted back into the knock-out variety of moss and its individual cell can be monitored under real time fluorescent microscope. This will demonstrate the localization of the target protein in the cells.


Related Solutions

The human gene for a protein hormone has been isolated. Scientists want to introduce this gene...
The human gene for a protein hormone has been isolated. Scientists want to introduce this gene into a bacterial host, modifying the bacteria so that it can be used to produce this hormone. After successfully introducing the gene on a plasmid, the bacteria produced the protein hormone, but the protein was defective, longer than normal, with intervening stretches of amino acids not found in the native protein. Which solution would be most likely to resolve this problem? Multiple Choice A....
Now you want to study the protein product of this mutant brat gene, and you want...
Now you want to study the protein product of this mutant brat gene, and you want to introduce this human brat gene into a bacterial expression vector (one that can be expressed to make a protein in bacterial cells) in hopes of producing a large amount of the human mutant Brat protein. Should you use the genomic DNA or the cDNA version of this gene? Explain your reasoning.
1) You are a research scientist studying a novel enzyme X, and you want to characterize...
1) You are a research scientist studying a novel enzyme X, and you want to characterize this new enzyme. You measure the velocity of the reaction with different substrate concentrations and get the following data: [S] (mM) Initial Velocity (mmol/min) 3.0 10.4 5.0 14.5 10.0 22.5 30.0 33.8 90.0 40.5 a) Graph the above data. From the graph, estimate KM and Vmax (Michaelis-Menten Plot) b) Use Lineweaver-Burk plot to calculate Km and Vmax. Show all equations and calculations. c) You...
what is the function of gene To perform vital tasks in cell, like transporting ions To...
what is the function of gene To perform vital tasks in cell, like transporting ions To contain information for building various cellular products To help cells move toward fuel and away from threats To allow cells to behave dynamically
Sterility is associated with loss of function (LOF) at a gene Z. The gene has 5000...
Sterility is associated with loss of function (LOF) at a gene Z. The gene has 5000 bases where LOF mutations can occur. Assuming the human mutation rate is 2x10^-8 per generation. Homozygotes for LOF alleles at Z are sterile, and heterozygotes have mild sterility issues. The heterozygotes for LOF alleles at Z have 20% fewer children than an average individual I the population, but suffer no other ill effects. A) What is the frequency of the Z allele under mutation-selection...
You discovered a novel antibiotic-resistant gene in a bacteria and would like to introduce the gene...
You discovered a novel antibiotic-resistant gene in a bacteria and would like to introduce the gene into a plant. Describe how you would clone and introduce the gene, and how you would determine whether each step was successful.
you want to purify 10g of a compound A that has been contaminated with 0.20g of...
you want to purify 10g of a compound A that has been contaminated with 0.20g of compound B. solubilities in water of the two compounds are given. compound A - solubility at 20°c= 0.029g/mol, solubility at 100°c= 0.680g/mol compound B - solubility at 20°c = 0.22, solubility at 100°c = 6.67 a) what volume of boiling water is needed to dissolve 10g of compound A? b) How much of compound A will remain after cooling to 20°c? c) what is...
You have three genes on the same chromosome - A, B and C. Each gene has...
You have three genes on the same chromosome - A, B and C. Each gene has two alleles in a dominant/recessive relationship. For these genes the homozygous recessive has the mutant phenotype for that trait, the dominant phenotype = wild type for that trait. allele A is dominant to a; phenotype a = mutant for trait a; phenotype A = wild type for trait A allele B is dominant to b; phenotype b = mutant for trait b; phenotype B...
You have three genes on the same chromosome - A, B and C. Each gene has...
You have three genes on the same chromosome - A, B and C. Each gene has two alleles in a dominant/recessive relationship. For these genes the homozygous recessive has the mutant phenotype for that trait, the dominant phenotype = wild type for that trait. allele A is dominant to a; phenotype a = mutant for trait a; phenotype A = wild type for trait A allele B is dominant to b; phenotype b = mutant for trait b; phenotype B...
You have three genes on the same chromosome - A, B and C. Each gene has...
You have three genes on the same chromosome - A, B and C. Each gene has two alleles in a dominant/recessive relationship. For these genes the homozygous recessive has the mutant phenotype for that trait, the dominant phenotype = wild type for that trait. allele A is dominant to a; phenotype a = mutant for trait a; phenotype A = wild type for trait A allele B is dominant to b; phenotype b = mutant for trait b; phenotype B...
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT