The sequence of a polypeptide X is as follows: THCARKEDSGYPEWANVRDECW 1. The Optical Density (O.D.) at...

The sequence of a polypeptide X is as follows:
THCARKEDSGYPEWANVRDECW

1. The Optical Density (O.D.) at 280 nm of a solution of the above polypeptide X is 0.51 at the standard experimental conditions (i.e. optical length L = 1.0 cm) . What is the concentration of the polypeptide X in this solution (in mM)? (The molar absorptivity (e) for tyrosine and tryptophan residues at 280 nm are 1,400 M^(-1)cm^(-1) and 5,500 M^(-1)cm^(-1), respectively).

2. Estimate the molecular weight of this peptide.

3. Draw side-chain structures and peptide bonds for the first five amino acid residues of the polypeptide X.

Expert Solution

queen_honey_blossom answered 5 months ago

What polypeptide sequence would you expect to be translated from the following unprocessed eukaryotic RNA sequence...

What polypeptide sequence would you expect to be translated from the following unprocessed eukaryotic RNA sequence 3ʼ AAG AUC UAA UAU UAC ACC GUA 5ʼ (the third and forth codons from the left are introns). Select one: a. N-terminus Met-Pro-His-Tyr- Asn-Leu-Glu C-terminus b. None of these answers is correct c. N-terminus Met-Pro-Asn-Leu-Glu C-terminus d. C-terminus Lys–Ile C-terminus e. N-terminus Lys-Ile C-terminus

You wish to determine the sequence of a polypeptide that has the following amino acid composition:...

You wish to determine the sequence of a polypeptide that has the following amino acid composition: Ala: 1 Arg: 4 Asn: 2 Asp: 3 Cys: 4 Gly: 3 Gln: 1 Glu: 4 His: 1 Lys :1 Met: 1 Phe: 1 Pro: 2 Ser: 4 Tyr: 2 Trp: 1 What is the maximum number of peptides you can expect if you cleave the polypeptide with: A) Trypsin B) Chymotrypsin C) CNBr D) There are no free sulfhydryl groups in the peptide....

. A double-stranded DNA molecule with the sequence shown here produces, in vivo, a polypeptide that...

. A double-stranded DNA molecule with the sequence shown here produces, in vivo, a polypeptide that is five amino acids long. TAC ATG ATC ATT TCA CGG AAT TTC TAG CAT GTA ATG TAC TAG TAA AGT GCC TTA AAG ATC GTA CAT a. Which strand of DNA is transcribed and in which direction? Support your response by indicating the 5 amino acid sequence that results when this transcript is translated. (2 pts) b. Label the 5’ and the 3’...

Suppose X follows a Gamma distribution with parameters α, β, and the following density function f(x)=...

Suppose X follows a Gamma distribution with parameters α, β, and the following density function f(x)= [x^(α−1)e^(−x/ β)]/ Γ(α)β^α . Find α and β so that E(X)= Var(X)=1. Also find the median for the random variable, X.

A random variable X has a density given by f X ( x ) = (1...

A random variable X has a density given by f X ( x ) = (1 − x^2 ) [ u ( x ) − u ( x − 1) ] + aδ ( x − 2 )where u(x) is the unit step function and δ(x) is a delta function. a). Find the value of a, E(X) and σ δx^2 . b). Find and make a labeled sketch of FX (x) . C). W = the event {X ≥ 0.5}....

1. Let X be random variable with density p(x) = x/2 for 0 < x<...

1. Let X be random variable with density p(x) = x/2 for 0 < x < 2 and 0 otherwise. Let Y = X^2−2. a) Compute the CDF and pdf of Y. b) Compute P(Y >0 | X ≤ 1.8).

Define a sequence from R as follows. Fix r > 1. Let a1 = 1 and...

Define a sequence from R as follows. Fix r > 1. Let a1 = 1 and define recursively, an+1 = (1/r) (an + r + 1). Show, by induction, that (an) is increasing and bounded above by (r+1)/(r−1) . Does the sequence converge?

The polypeptide sequence below has three continuous regions in which the residues have similar chemical properties....

The polypeptide sequence below has three continuous regions in which the residues have similar chemical properties. Identify these and very briefly describe their properties: DKEKRESNQYTMLVFI

#You have the sequence 5' AUG AAG UUC UGA. Write the corresponding polypeptide Now do...

#You have the sequence 5' AUG AAG UUC UGA. Write the corresponding polypeptide Now do the following operations: a) change any one nucleotide (type it in bold face) b) add a single nucleotide at any position to the original sequence (again type it in bold face) c) remove a single nucleotide at any position to the original sequence (again type it in bold face) write down the corresponding polypeptides. Which change(s) affected the polypeptide...

1) Define a sequence of polynomials H n (x ) by H 0 (x )=1, H...

1) Define a sequence of polynomials H n (x ) by H 0 (x )=1, H 1 (x )=2 x , and for n>1 by H n+1 (x )=2 x H n (x )−2 n H n−1 (x ) . These polynomials are called Hermite polynomials of degree n. Calculate the first 7 Hermite polynomials of degree less than 7. You can check your results by comparing them to the list of Hermite polynomials on wikipedia (physicist's Hermite polynomials). 2)...

Question