The sequence of a polypeptide X is as follows: THCARKEDSGYPEWANVRDECW 1. The Optical Density (O.D.) at...

The sequence of a polypeptide X is as follows:
THCARKEDSGYPEWANVRDECW

1. The Optical Density (O.D.) at 280 nm of a solution of the above polypeptide X is 0.51 at the standard experimental conditions (i.e. optical length L = 1.0 cm) . What is the concentration of the polypeptide X in this solution (in mM)? (The molar absorptivity (e) for tyrosine and tryptophan residues at 280 nm are 1,400 M^(-1)cm^(-1) and 5,500 M^(-1)cm^(-1), respectively).

2. Estimate the molecular weight of this peptide.

3. Draw side-chain structures and peptide bonds for the first five amino acid residues of the polypeptide X.

Solutions

Expert Solution

queen_honey_blossom answered 2 weeks ago

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