In: Chemistry
The sequence of a polypeptide X is as follows:
THCARKEDSGYPEWANVRDECW
1. The Optical Density (O.D.) at 280 nm of a solution of the above
polypeptide X is 0.51 at the standard experimental conditions (i.e.
optical length L = 1.0 cm) . What is the concentration of the
polypeptide X in this solution (in mM)? (The molar absorptivity (e)
for tyrosine and tryptophan residues at 280 nm are 1,400
M^(-1)cm^(-1) and 5,500 M^(-1)cm^(-1), respectively).
2. Estimate the molecular weight of this peptide.
3. Draw side-chain structures and peptide bonds for the first five
amino acid residues of the polypeptide X.