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In: Biology

Some organisms require oxygen in significantly reduced amounts, such as 5% oxygen. These organisms are classified...

Some organisms require oxygen in significantly reduced amounts, such as 5% oxygen. These organisms are classified as

	A.	obligate aerobes.
	B.	obligate anaerobes.
	C.	microaerophiles.
	D.	facultative anaerobes.

he most critical step in the Gram stain technique involves what reagent?

A.

Crystal violet

B.

Iodine

C.

Alcohol

D.

Safranin

Spirit blue agar is used to test for

	A.	amylase.
	B.	proteolysis
	C.	indole production.
	D.	lipase

What product is detected when testing for the presence of tryptophanase?

A.

Indole

B.

Ammonia

C.

Tryptophan

D.

Pyruvic acid

Expert Solution

1 OBLIGATE ANAEROBES ARE MICROORGANISM KILLED BY NORMAL ATMOSPHERIC CONCENTRATION OF OXYGEN

MICROAEROPHILES ,LIKE OBLIGATE ANAEROBES DAMAGED BY NORMAL ATMOSPHERIC CONCENTRATION OF OXYGEN .BUT THEY METABOLISE ENERGY AEROBICALLY BUT OBLIGATE ANAEROBES METABOLISE ENERGY ANAEROBICALLY .MICROAEROPHILES THEREFORE REQUIRE OXYGEN 2 TO 10 PERCENTAGE FOR GROWTH .BUT OBLIGATE ANAEROBES DO NOT

OBLIGATE AEROBES NEED OXYGEN

FACULTATIVE ANAEROBES CAN GROW WITH OR WITH OUT OXYGEN

ANSWER C MICROAEROPHILES

2 GRAM STAINING HAS FOUR BASIC STEPS

APPLYING PRIMARY STAIN CRYSTAL VIOLET TO A HEAT FIXED SMEAR

ADDITION OF A MORDANT LIKE GRAMS IODINE

RAPID RECOLORIZATION WITH ALCOHOL ,ACETONE OR A MIXTURE OF BOTH

COUNTER STAINING WITH SAFRANIN

THE CRITICAL STEP IN GRAMSTAINING IS DECOLORIZATION AND THE REAGENT IS ALCOHOL

3 SPIRIT BLUE AGAR IS USED TO IDENTIFY ORGANISM THAT ARE CAPABLE OF PRODUCING ENZYME LIPASE

4 SPOT INDOLE TEST IS USED TO DETERMINE THE PRESENCE OF ENZYME TRYPTOPHANASE . TRYPTOPHANASE BREAKS DOWN TRYPTOPHAN TO RELEASE INDOLE

gladiator answered 2 years ago

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