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Beers Law Lab CuSO4 sol. ranging from 0.00M to 0.40M is prepared to record percentage transmission...

Beers Law

Lab
CuSO4 sol. ranging from 0.00M to 0.40M is prepared to record percentage transmission through spectrometer. Since 0.40M is quite high to obey beers law, but still I got linear graph. Why it's still linear??

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Expert Solution

Yes, it is true that any solution obeys Beers law within a certain range of the concentration of a particular solution. There are many reasons, below are few: due to electrostatic interactions between molecules in close proximity affects the %Transmission >scattering of light due to particulates in the sample: As concentration increases scattering of light also increases and this will affect on %T. > fluorescence or phosphorescence of the sample: may also affect on %T. >changes in the refractive index at high concentration may affect %T.

Thus the concentration range of a solution which can obey Beers law can be dependent on many factors. 0.40M concentration may fall within the rang which obeys the beers law with that particular instrument with a specific type of glass cuvette you using. If rest of all %T readings are falling on the same line means with given conditions solution is still following Beers law.


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