In: Chemistry
Beers Law
Lab
CuSO4 sol. ranging from 0.00M to 0.40M is prepared to record
percentage transmission through spectrometer. Since 0.40M is quite
high to obey beers law, but still I got linear graph. Why it's
still linear??
Yes, it is true that any solution obeys Beers law within a certain range of the concentration of a particular solution. There are many reasons, below are few: due to electrostatic interactions between molecules in close proximity affects the %Transmission >scattering of light due to particulates in the sample: As concentration increases scattering of light also increases and this will affect on %T. > fluorescence or phosphorescence of the sample: may also affect on %T. >changes in the refractive index at high concentration may affect %T.
Thus the concentration range of a solution which can obey Beers law can be dependent on many factors. 0.40M concentration may fall within the rang which obeys the beers law with that particular instrument with a specific type of glass cuvette you using. If rest of all %T readings are falling on the same line means with given conditions solution is still following Beers law.